Liu Xiangyan, Zhang Fusheng, Li Mengyao, Li Rong, Zhang Zhen, Xu Juan, Wen Lixin, Li Rongfang
Hunan Engineering Research Center of Livestock and Poultry Health Care, College of Veterinary Medicine, Hunan Agricultural University, Changsha, China.
Changsha Lvye Biotechnology Co., Ltd., Changsha, China.
Front Vet Sci. 2024 Aug 21;11:1417309. doi: 10.3389/fvets.2024.1417309. eCollection 2024.
(vine tea), a high polyphenol content antioxidant plant resource, is renowned for its medicinal benefits. This study aimed to investigate the effects of extract (AGE) on anti-inflammatory and antioxidant ability, enhancement of intestinal immunity, improvement of intestinal structure, and regulation of gut microbiota in swine.
A total of 135 weaned piglets were randomly divided into three groups: a control group, a low-dose group, and a high-dose group. Pigs were weighed and blood was collected on days 36, 85, and 154. The feed intake was recorded daily to calculate growth performance parameters. On day 154, five to six pigs in each group were randomly selected and euthanized to obtain a small intestine to investigate the effects of AGE on anti-inflammatory and antioxidant abilities and gut microbiota.
The results showed that 500 mg/kg AGE increased the expression of anti-inflammatory and immune cytokines (IL-10, IgG, and IgA) ( < 0.05, < 0.01) and decreased the expression of proinflammatory cytokines (IL-1β) ( < 0.05) in serum. Additionally, 500 mg/kg AGE enhanced the antioxidant capacity by increasing the GSH-Px, CAT, and SOD ( < 0.05, < 0.01).
A total of 500 mg/kg AGE significantly increased the abundance of gut microbiota, enhanced the gut barrier, and modulated gut immunity. During the piglet phase, 500 mg/kg AGE increased the relative abundance of ( < 0.05). During the growing-finishing phase, 500 mg/kg AGE increased the relative abundance of and ( < 0.05, < 0.01). Overall, we recommended 500 mg/kg AGE as a routine addition dose for swine to improve porcine growth performance and intestinal health.
藤茶是一种富含多酚的抗氧化植物资源，以其药用价值而闻名。本研究旨在探讨藤茶提取物（AGE）对猪的抗炎和抗氧化能力、增强肠道免疫力、改善肠道结构以及调节肠道微生物群的影响。
将135头断奶仔猪随机分为三组：对照组、低剂量组和高剂量组。在第36、85和154天对猪进行称重并采集血液。每天记录采食量以计算生长性能参数。在第154天，每组随机选取五至六头猪进行安乐死，获取小肠以研究AGE对抗炎和抗氧化能力以及肠道微生物群的影响。
结果表明，500mg/kg AGE可增加血清中抗炎和免疫细胞因子（IL-10、IgG和IgA）的表达（P<0.05，P<0.01），并降低促炎细胞因子（IL-1β）的表达（P<0.05）。此外，500mg/kg AGE通过增加GSH-Px、CAT和SOD来增强抗氧化能力（P<0.05，P<0.01）。
500mg/kg AGE显著增加了肠道微生物群的丰度，增强了肠道屏障，并调节了肠道免疫力。在仔猪阶段，500mg/kg AGE增加了[具体菌属]的相对丰度（P<0.05）。在生长育肥阶段，500mg/kg AGE增加了[具体菌属]和[具体菌属]的相对丰度（P<0.05，P<0.01）。总体而言，我们建议将500mg/kg AGE作为猪的常规添加剂量，以改善猪的生长性能和肠道健康。
