Mey Gabrielle M, Shelestak John, Irfan Muhammad, DeSilva Tara M
Department of Ophthalmology and Visual Sciences, University of Michigan, Michigan Medicine, Ann Arbor, MI 48105, USA; Michigan Neuroscience Institute, University of Michigan, Michigan Medicine, Ann Arbor, MI 48105, USA.
Department of Neurosciences, Cleveland Clinic Lerner College of Medicine and Case Western Reserve University, Cleveland, OH 44195, USA.
STAR Protoc. 2025 Jun 20;6(2):103860. doi: 10.1016/j.xpro.2025.103860. Epub 2025 Jun 4.
Visual system pathology has been established as a prominent feature in demyelinating diseases such as multiple sclerosis (MS). Here, we present a protocol for assessing regional pathology in the rodent optic nerve using longitudinal cryosections and cross-sectional electron microscopy. We describe steps for optic nerve dissection, post-fixation, myelin detection, slide scanning of myelin basic protein, and the analysis of demyelination. We then detail procedures for fluorescent antibody staining, analysis for transmission electron microscopy (TEM), and the analysis of myelinated and unmyelinated axons. For complete details on the use and execution of this protocol, please refer to Mey et al..
视觉系统病理学已被确认为多发性硬化症（MS）等脱髓鞘疾病的一个显著特征。在此，我们介绍一种使用纵向冰冻切片和横断面电子显微镜评估啮齿动物视神经区域病理学的方案。我们描述了视神经解剖、固定后处理、髓鞘检测、髓鞘碱性蛋白的玻片扫描以及脱髓鞘分析的步骤。然后，我们详细说明了荧光抗体染色、透射电子显微镜（TEM）分析以及有髓和无髓轴突分析的程序。有关本方案使用和执行的完整详细信息，请参考Mey等人的研究。
