Chen Xipeng, Cheng Yuanpei, Li Yongbo, Tan Ze, Wu Han
Department of Orthopedics, China-Japan Union Hospital of Jilin University, Changchun, 130033, China.
Shandong First Medical University Affiliated Provincial Hospital, Jinan, 250100, China.
Mater Today Bio. 2025 May 7;32:101847. doi: 10.1016/j.mtbio.2025.101847. eCollection 2025 Jun.
The clinical management of infected bone defects remains a significant challenge in orthopedic surgery. In this study, we developed a multifunctional composite scaffold by integrating MXene (TiC) nanosheets and vancomycin (Van) into a degradable poly(glycolide-co-caprolactone) (PGCL) polymer through precision 3D printing technology, with the aim of regenerating acutely infected bone defects. Systematic optimization established the optimal composition as 5 wt% TiC and 5 wt% Van within the degradable PGCL polymer. Compared with PGCL scaffolds, a significantly thicker mineralized layer could be deposited on the PGCL@5 %TiC/5 %Van surface in simulated body fluid (SPF). The results showed that the PGCL@5 %TiC/5 %Van scaffold had antibacterial properties, cytocompatibility, osteoblast differentiation and extracellular mineralization. The results showed that the PGCL@5 %Van/5 %TiC scaffold effectively inhibited infection caused by methicillin-resistant in bone defects and promoted bone repair. The potential mechanism of TiC in anti-inflammatory and osteogenic induction was investigated through transcriptome analysis. These results suggest that TiC is involved in the regulation of the PI3K‒Akt signalling pathway to increase osteogenic induction ability and regulate the inflammatory response by downregulating the NF-κB pathway through the deiodinase iodothyronine type II (DiO2). These findings indicate that the PGCL@5 %TiC/5 %Van scaffold can enhance the regeneration of acutely infected bone defects.
感染性骨缺损的临床管理仍是骨科手术中的一项重大挑战。在本研究中，我们通过精密3D打印技术将MXene（TiC）纳米片和万古霉素（Van）整合到可降解的聚（乙交酯-共-己内酯）（PGCL）聚合物中，开发了一种多功能复合支架，旨在再生急性感染性骨缺损。系统优化确定了可降解PGCL聚合物中5 wt% TiC和5 wt% Van为最佳组成。与PGCL支架相比，在模拟体液（SPF）中，PGCL@5%TiC/5%Van表面可沉积明显更厚的矿化层。结果表明，PGCL@5%TiC/5%Van支架具有抗菌性能、细胞相容性、成骨细胞分化和细胞外矿化能力。结果表明，PGCL@5%Van/5%TiC支架可有效抑制骨缺损中耐甲氧西林金黄色葡萄球菌引起的感染并促进骨修复。通过转录组分析研究了TiC在抗炎和成骨诱导中的潜在机制。这些结果表明，TiC通过II型脱碘酶（DiO2）下调NF-κB途径，参与PI3K-Akt信号通路的调节，以增加成骨诱导能力并调节炎症反应。这些发现表明，PGCL@5%TiC/5%Van支架可增强急性感染性骨缺损的再生。
