Glutathione peroxidase 3 antagonizes oxidative stress and inflammation in diabetic retinopathy via NrF2/NF-κB pathway.

PURPOSE

Hyperglycemia-oxidative stress (OS)-inflammation-apoptosis cascade reaction is an important pathogenesis of diabetes retinopathy (DR). However, the function of glutathione peroxidase 3 (GPX3), an important antioxidant molecule, in the pathogenesis of DR remains unclear. This study aims to explore the function and mechanism of GPX3 mediated OS in the occurrence and development of DR.

METHODS

Human retinal capillary endothelial cells (HRCECs) were cultured in vitro to construct a high glucose environment, GPX3 deficiency, and GPX3 overexpression HRCEC cell model. Cell proliferation activity, OS level, inflammation, apoptosis, and NrF2/NF-κB pathway expression were detected by CCK-8 assay, qRT-PCR, Western blot, and flow cytometry.

RESULTS

The high glucose treatment resulted in a time and dose dependent decrease in the proliferation activity and GPX3 expression level of HRCECs, and promoted the occurrence of cell OS, inflammation, and apoptosis. At the same time, it inhibited the expression of nuclear factor erythroid 2-related factor 2 (NrF2) and up-regulated the expression of nuclear factor kappa B (NF-κB); The down-regulation of GPX3 expression exacerbated the effects of high glucose environment on the OS, inflammation, apoptosis, and NrF2/NF-κB pathway of HRCECs, while overexpression of GPX3 can partially antagonize the effects of high glucose environment on the OS, inflammation, apoptosis, and NrF2/NF-κB pathway of HRCECs.

CONCLUSIONS

Targeted regulation of GPX3, which is down-regulated and inactivated in DR, is expected to mediate the occurrence and development of OS, inflammation, and cell apoptosis in DR through the NrF2/NF-κB pathway.