Wei Ziqi, Peng Zhen, Li Junyi, Tang Hui, Qiao Baoling, Madjirebaye Philippe, Xiong Tao
State Key Laboratory of Food Science and Resources, Nanchang University, 235 Nanjing East Road, Nanchang, 330047, Jiangxi Province, China.
International Institute of Food Innovation Co., Ltd., Nanchang University, Nanchang, 330200, Jiangxi Province, China.
Probiotics Antimicrob Proteins. 2025 Jun 7. doi: 10.1007/s12602-025-10620-4.
Helicobacter pylori (H. pylori) is the leading cause of infectious gastritis, and its resistance to antibiotics is becoming increasingly significant. Probiotics have been widely reported to have antagonistic effects on H. pylori. In this study, 52 strains of lactic acid bacteria (LAB) were isolated from the feces of healthy human and animal. The acid tolerance, anti-H. pylori ability, urease inhibition rate, and nickel adsorption rate of 19 LAB were determined. PCA evaluation revealed Lactiplantibacillus plantarum (L. plantarum) NCUA001057, Pediococcus pentosaceus (P. pentosaceus) NCUH006018, and L. plantarum NCUH001090 displaying the best comprehensive performance. Subsequently, the hemolytic, bile salt tolerance, antioxidant capacity, co-aggregation ability with H. pylori, and adhesion rate to AGS cells of three LAB were evaluated. Moreover, three LAB downregulated the production of IL-1β and IL-8 in H. pylori pre-treated AGS cells. We preliminarily studied the antibacterial substances of three LAB which mainly included acid, hydrogen peroxide, and protein, and the antibacterial substances are heat-tolerant. Furthermore, we found that the main modes of antibacterial action of P. pentosaceus NCUH006018 were as follows: inhibited the adhesion of H. pylori to AGS cells, made H. pylori spherical and destroyed its intact cell structure, and downregulated the metabolism genes of H. pylori flagellin and ribosome. In this study, the high potential of antagonizing H. pylori by three novel LAB was demonstrated, and their mode of antibacterial action provided a new strategy for prevention or inhibition of H. pylori infection.
幽门螺杆菌（H. pylori）是感染性胃炎的主要病因，其对抗生素的耐药性日益显著。益生菌对幽门螺杆菌具有拮抗作用，这已得到广泛报道。在本研究中，从健康人和动物的粪便中分离出52株乳酸菌（LAB）。测定了19株乳酸菌的耐酸性、抗幽门螺杆菌能力、脲酶抑制率和镍吸附率。主成分分析（PCA）评估显示，植物乳杆菌（L. plantarum）NCUA001057、戊糖片球菌（P. pentosaceus）NCUH006018和植物乳杆菌NCUH001090表现出最佳的综合性能。随后，对这三株乳酸菌的溶血能力、胆盐耐受性、抗氧化能力、与幽门螺杆菌的共聚集能力以及对AGS细胞的黏附率进行了评估。此外，这三株乳酸菌下调了幽门螺杆菌预处理的AGS细胞中白细胞介素-1β（IL-1β）和白细胞介素-8（IL-8）的产生。我们初步研究了这三株乳酸菌的抗菌物质，主要包括酸、过氧化氢和蛋白质，且这些抗菌物质耐热。此外，我们发现戊糖片球菌NCUH006018的主要抗菌作用方式如下：抑制幽门螺杆菌对AGS细胞的黏附，使幽门螺杆菌呈球形并破坏其完整的细胞结构，下调幽门螺杆菌鞭毛蛋白和核糖体的代谢基因。本研究证明了三株新型乳酸菌对幽门螺杆菌具有很高的拮抗潜力，其抗菌作用方式为预防或抑制幽门螺杆菌感染提供了新策略。
