Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China; Science Center for Future Foods, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China; Engineering Research Center of Ministry of Education on Food Synthetic Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China; Jiangsu Province Engineering Research Center of Food Synthetic Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China.
Fujian Maidu Food Development Co., Ltd, Quanzhou, Fujian 362000, China.
Sci Total Environ. 2024 Jan 1;906:167587. doi: 10.1016/j.scitotenv.2023.167587. Epub 2023 Oct 4.
Listeria monocytogenes is one of the most lethal foodborne pathogens, and there is a lack of microorganisms that can strongly inhibit its growth. Safe lactic acid bacteria with probiotic and antibacterial properties are ideal sources of antagonistic bacteria. This study isolated a strain of Lactobacillus plantarum 4-10 that completely killed L. monocytogenes from northeastern Chinese sauerkraut. Probiotic characterization revealed broad-spectrum bacterial inhibition, antagonizing 16 Gram-positive, Gram-negative, and fungal species. After tolerance to simulated intestinal and gastric fluids, the survival rate was >45 %. L. plantarum 4-10 was sensitive to chloramphenicol, doxycycline, erythromycin, and tetracycline, and exhibited good hydrophobicity, auto-aggregation, and co-aggregation. It could disrupt the cell structure when co-cultured with L. monocytogenes and act as a lethal agent within 15 h. Through transcriptomic analysis and validation experiments, we found that L. plantarum 4-10 could inhibit the expression of L. monocytogenes membrane transport-related genes by producing bacteriocins, thus disrupting the cell membrane structure and inhibiting the growth, metabolic viability, and biofilm formation of L. monocytogenes in a short time. In conclusion, L. plantarum 4-10 has good probiotic properties and antibacterial effects and shows excellent research and application prospects as a natural bacteriostat.
单增李斯特菌是最致命的食源性病原体之一，而能够强烈抑制其生长的微生物却很缺乏。具有益生菌和抗菌特性的安全乳酸菌是理想的拮抗细菌来源。本研究从中国东北酸菜中分离到一株完全杀死单核细胞增生李斯特菌的植物乳杆菌 4-10。益生菌特性分析显示其具有广谱抑菌作用，可拮抗 16 种革兰氏阳性菌、革兰氏阴性菌和真菌。经过模拟肠道和胃液的耐受后，存活率>45%。植物乳杆菌 4-10 对氯霉素、强力霉素、红霉素和四环素敏感，表现出良好的疏水性、自聚集和共聚集能力。与单核细胞增生李斯特菌共培养时可破坏其细胞结构，并在 15 小时内充当致死剂。通过转录组分析和验证实验，我们发现植物乳杆菌 4-10 可以通过产生细菌素抑制单核细胞增生李斯特菌的膜转运相关基因的表达，从而在短时间内破坏细胞膜结构，抑制单核细胞增生李斯特菌的生长、代谢活力和生物膜形成。总之，植物乳杆菌 4-10 具有良好的益生菌特性和抗菌效果，作为一种天然抑菌剂具有优异的研究和应用前景。
