Sanner H E, Wooten M J
Transfusion. 1985 Sep-Oct;25(5):437-8. doi: 10.1046/j.1537-2995.1985.25586020119.x.
A protocol was developed under which washed red cells were used to provide small-volume transfusions for neonatal patients (under 4 months of age). Two pediatric units, each containing approximately 135 ml of red cells, were prepared from a freshly collected unit of group O Rh-negative whole blood. Units not more than 6 days old were washed with 1l of 0.9 percent NaCl in a blood cell processor. Aliquots of the washed red cells were drawn into labeled syringes for transfusion to neonatal patients. Data collected from 40 units of washed red cells prepared according to this protocol showed 83 percent red cell recovery, and satisfactory reduction in white cells, plasma proteins, extracellular potassium, red cell metabolic waste products, and anticoagulant. Over 5 years, a total of 2085 different neonatal patients received 7875 separate transfusions prepared from 1860 units of washed red cells. The clinical response was excellent, and no suspected reactions were reported.
制定了一项方案，在此方案下，使用洗涤红细胞为新生儿患者（4个月以下）提供小容量输血。从新鲜采集的1单位O型Rh阴性全血制备了两个儿科单位，每个单位含有约135毫升红细胞。不超过6天的单位在血细胞处理器中用1升0.9%氯化钠洗涤。将洗涤后的红细胞等分试样吸入标记的注射器中，用于输给新生儿患者。根据该方案制备的40单位洗涤红细胞的数据显示，红细胞回收率为83%，白细胞、血浆蛋白、细胞外钾、红细胞代谢废物和抗凝剂均有令人满意的减少。在5年多的时间里，共有2085名不同的新生儿患者接受了由1860单位洗涤红细胞制备的7875次单独输血。临床反应良好，未报告疑似反应。
