Shahbazi Sanaz, Ghaffarian Sara, Razinataj Mohammad, Zangi Mohammad Reza, Hamid Rasmieh, Panahi Bahman
Department of Cellular and Molecular Biology, Faculty of Sciences, Azerbaijan Shahid Madani University, Tabriz, Iran.
Department of Plant Protection, Cotton Research Institute of Iran (CRII), Agricultural Research, Education and Extension Organization (AREEO), Gorgan, Iran.
Biochem Biophys Rep. 2025 May 19;42:102059. doi: 10.1016/j.bbrep.2025.102059. eCollection 2025 Jun.
Verticillium wilt (VW) is one of the most devastating diseases affecting cotton ( spp.), causing significant yield losses worldwide. The development of resistant cultivars is a primary strategy for managing this disease; however, conventional breeding approaches often encounter challenges in balancing resistance with high yield potential. This study aimed to assess the genetic diversity of 25 cotton cultivars using simple sequence repeat (SSR) markers and to identify key polymorphic markers associated with VW resistance. A total of 16 SSR markers were utilized, of which five (DPL405, DPL752, DPL866, DPL890, and DPL0022) were polymorphic. The polymorphism information content (PIC) values ranged from 0 to 0.49, with DPL405, DPL866, and DPL890 being the most informative. Principal coordinates analysis (PCoA) demonstrated genetic differentiation between resistant and sensitive cultivars, with the first axis explaining 41.19 % of the total variation. Resistant cultivars such as Leader, Golestan, and Arya clustered distinctly from sensitive varieties, confirming the effectiveness of the selected markers in genetic differentiation. Despite the promising results, key limitations include the relatively low overall marker polymorphism and the limited number of SSRs used, which may constrain broader genomic coverage and resolution. Nonetheless, the findings provide valuable insights for cotton breeding programs and highlight the potential of SSR markers in supporting marker-assisted selection (MAS) for Verticillium wilt resistance.
黄萎病是影响棉花（品种）的最具毁灭性的病害之一，在全球范围内造成重大产量损失。培育抗病品种是防治这种病害的主要策略；然而，传统育种方法在平衡抗性与高产潜力方面常常面临挑战。本研究旨在利用简单序列重复（SSR）标记评估25个棉花品种的遗传多样性，并鉴定与黄萎病抗性相关的关键多态性标记。共使用了16个SSR标记，其中5个（DPL405、DPL752、DPL866、DPL890和DPL0022）具有多态性。多态性信息含量（PIC）值范围为0至0.49，其中DPL405、DPL866和DPL890信息量最大。主坐标分析（PCoA）表明抗病品种和感病品种之间存在遗传分化，第一轴解释了总变异的41.19%。像Leader、Golestan和Arya等抗病品种与感病品种明显聚类，证实了所选标记在遗传分化中的有效性。尽管取得了有希望的结果，但关键限制包括总体标记多态性相对较低以及使用的SSR数量有限，这可能会限制更广泛的基因组覆盖范围和分辨率。尽管如此，这些发现为棉花育种计划提供了有价值的见解，并突出了SSR标记在支持黄萎病抗性标记辅助选择（MAS）方面的潜力。
