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Biology (Basel). 2012 Sep 25;1(3):460-83. doi: 10.3390/biology1030460.
The advent of next-generation DNA sequencing (NGS) technologies has led to the development of rapid genome-wide Single Nucleotide Polymorphism (SNP) detection applications in various plant species. Recent improvements in sequencing throughput combined with an overall decrease in costs per gigabase of sequence is allowing NGS to be applied to not only the evaluation of small subsets of parental inbred lines, but also the mapping and characterization of traits of interest in much larger populations. Such an approach, where sequences are used simultaneously to detect and score SNPs, therefore bypassing the entire marker assay development stage, is known as genotyping-by-sequencing (GBS). This review will summarize the current state of GBS in plants and the promises it holds as a genome-wide genotyping application.
下一代 DNA 测序（NGS）技术的出现，使得在各种植物物种中快速进行全基因组单核苷酸多态性（SNP）检测的应用成为可能。测序通量的最新改进，加上每千兆碱基序列成本的总体下降，使得 NGS 不仅可以应用于小部分亲本自交系的评估，还可以应用于更大群体中感兴趣性状的定位和特征描述。这种方法是同时使用序列来检测和评分 SNP，从而绕过整个标记检测开发阶段，被称为测序基因型分析（GBS）。本文综述了 GBS 在植物中的现状及其作为全基因组基因型分析应用的前景。
