Rotunjanu Slaviţa, Racoviceanu Roxana, Gogulescu Armand, Mioc Alexandra, Milan Andreea, Marangoci Narcisa Laura, Dascălu Andrei-Ioan, Mioc Marius, Negrea-Ghiulai Roxana, Trandafirescu Cristina, Șoica Codruţa
Department of Pharmacology-Pharmacotherapy, Faculty of Pharmacy, Victor Babes University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timișoara, Romania.
Research Center for Experimental Pharmacology and Drug Design (X-Pharm Design), Victor Babes University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timișoara, Romania.
Nanomaterials (Basel). 2025 May 30;15(11):829. doi: 10.3390/nano15110829.
In this study, praseodymium-doped cobalt ferrite nanoparticles (CoFePrO, y = 0-0.2) were synthesized via sol-gel auto-combustion and systematically characterized to assess their structural, morphological, magnetic, and biological properties. X-ray diffraction (XRD) confirmed single-phase cubic cobalt ferrite formation for samples with y ≤ 0.05 and the emergence of a secondary orthorhombic PrFeO phase at higher dopant concentrations. FTIR spectroscopy identified characteristic metal-oxygen vibrations and revealed a progressive shift of absorption bands with increasing praseodymium (Pr) content. Vibrating sample magnetometry (VSM) demonstrated a gradual decline in saturation (Ms) and remanent (Mr) magnetization with Pr doping, an effect further intensified by cyclodextrin surface coating. TEM analyses revealed a particle size increase correlated with dopant level, while SEM images displayed a porous morphology typical of combustion-synthesized ferrites. In vitro cell viability assays showed minimal toxicity in normal human keratinocytes (HaCaT), while significant antiproliferative activity was observed against human cancer cell lines A375 (melanoma), MCF-7 (breast adenocarcinoma), and HT-29 (colorectal adenocarcinoma), particularly in Pr 6-CD and Pr 7-CD samples. These findings suggest that Pr substitution and cyclodextrin coating can effectively modulate the physicochemical and anticancer properties of cobalt ferrite nanoparticles, making them promising candidates for future biomedical applications.
在本研究中，通过溶胶-凝胶自燃法合成了掺镨钴铁氧体纳米颗粒（CoFePrO，y = 0 - 0.2），并对其结构、形态、磁性和生物学性质进行了系统表征。X射线衍射（XRD）证实，y≤0.05的样品形成单相立方钴铁氧体，而在较高掺杂浓度下会出现次生正交PrFeO相。傅里叶变换红外光谱（FTIR）确定了特征性的金属-氧振动，并揭示了随着镨（Pr）含量增加吸收带的逐渐位移。振动样品磁强计（VSM）表明，Pr掺杂会使饱和磁化强度（Ms）和剩余磁化强度（Mr）逐渐下降，环糊精表面包覆会进一步增强这种效应。透射电子显微镜（TEM）分析表明粒径随掺杂水平增加，而扫描电子显微镜（SEM）图像显示出燃烧合成铁氧体典型的多孔形态。体外细胞活力测定表明，在正常人角质形成细胞（HaCaT）中毒性极小，而对人癌细胞系A375（黑色素瘤）、MCF-7（乳腺腺癌）和HT-29（结肠腺癌）具有显著的抗增殖活性，尤其是在Pr 6-CD和Pr 7-CD样品中。这些发现表明，Pr取代和环糊精包覆可以有效调节钴铁氧体纳米颗粒的物理化学和抗癌性能，使其成为未来生物医学应用的有前途的候选材料。
