Petru Andreea-Elena, Iacovita Cristian, Fizeșan Ionel, Dudric Roxana, Crestin Ionut-Valentin, Lucaciu Constantin Mihai, Loghin Felicia, Kiss Bela
Department of Toxicology, Faculty of Pharmacy, "Iuliu Hațieganu" University of Medicine and Pharmacy, Pasteur 6A, 400349 Cluj-Napoca, Romania.
Department of Pharmaceutical Physics-Biophysics, Faculty of Pharmacy, "Iuliu Hatieganu" University of Medicine and Pharmacy, Pasteur 6, 400349 Cluj-Napoca, Romania.
Pharmaceutics. 2025 Mar 18;17(3):384. doi: 10.3390/pharmaceutics17030384.
: Magnetic hyperthermia (MH) has emerged as a promising alternative to conventional cancer treatments, offering targeted tumor destruction with minimal damage to healthy tissues. In this study, we synthesized manganese-doped magnetic nanoflowers (Mn-NFs) using a polyol-mediated approach to enhance heating efficiency and biocompatibility for MH applications. Our objective was to evaluate their structural, magnetic, and in vitro hyperthermic properties to determine their potential for lung cancer therapy. : Mn-NFs, with the general formula MnxFe-xO (x = 0, 0.3, 0.5, 0.7), were synthesized via a one-step polyol method and characterized using transmission electron microscopy (TEM), X-ray diffraction (XRD), and vibrating sample magnetometry (VSM). Their heating efficiency was assessed through specific absorption rate (SAR) measurements in aqueous and solid environments under an alternating magnetic field (AMF). Cytocompatibility was evaluated using the Alamar Blue assay on A549 lung carcinoma cells. Cellular uptake was quantified via a colorimetric iron determination method, while in vitro MH efficacy was tested by subjecting Mn-NF-loaded A549 cells to AMF exposure at different field strengths and nanoparticle concentrations. : Mn-NFs exhibited a flower-like morphology with enhanced magnetic properties, achieving high SAR values, particularly in immobilized conditions. Cytotoxicity assays confirmed high biocompatibility at relevant doses, with Mn-NFs of x = 0.3 showing optimal cellular uptake. MH studies demonstrated significant cancer cell death at AMF intensities of around 30 kA/m, with increased effectiveness following static magnetic field pre-alignment. : The results highlight Mn-NFs, particularly those with a Mn content of x = 0.3, as promising candidates for MH-based lung cancer therapy, combining high heating efficiency, biocompatibility, and effective intracellular uptake. Further studies are needed to validate their therapeutic potential in vivo.
磁热疗（MH）已成为传统癌症治疗方法的一种有前景的替代方案，能够在对健康组织损伤最小的情况下实现靶向肿瘤破坏。在本研究中，我们采用多元醇介导的方法合成了锰掺杂磁性纳米花（Mn-NFs），以提高热疗应用中的加热效率和生物相容性。我们的目标是评估其结构、磁性和体外热疗特性，以确定它们在肺癌治疗中的潜力。：通过一步多元醇法合成了通式为MnxFe-xO（x = 0, 0.3, 0.5, 0.7）的Mn-NFs，并使用透射电子显微镜（TEM）、X射线衍射（XRD）和振动样品磁强计（VSM）对其进行表征。通过在交变磁场（AMF）下在水性和固体环境中测量比吸收率（SAR）来评估它们的加热效率。使用Alamar Blue检测法对A549肺癌细胞进行细胞相容性评估。通过比色法铁测定法对细胞摄取进行定量，同时通过在不同场强和纳米颗粒浓度下对负载Mn-NF的A549细胞进行AMF暴露来测试体外MH疗效。：Mn-NFs呈现出具有增强磁性的花状形态，实现了高SAR值，尤其是在固定条件下。细胞毒性试验证实了在相关剂量下具有高生物相容性，x = 0.3的Mn-NFs表现出最佳的细胞摄取。MH研究表明，在AMF强度约为30 kA/m时癌细胞有显著死亡，在静态磁场预排列后有效性增加。：结果突出了Mn-NFs，特别是那些Mn含量为x = 0.3的Mn-NFs，作为基于MH的肺癌治疗的有前景候选物，它们兼具高加热效率、生物相容性和有效的细胞内摄取。需要进一步研究以验证它们在体内的治疗潜力。
