Rossez Yannick, Léonard Renaud, Vidal Olivier, Chouit Zina, Guerardel Yann, Madec Edwige, Robbe-Masselot Catherine
Faculté des sciences et technologies, UMR 8576 CNRS, Unité de glycobiologie structurale et fonctionnelle, Villeneuve d'Ascq, France.
Methods Mol Biol. 2025;2942:177-186. doi: 10.1007/978-1-0716-4627-4_15.
The handling of high-molecular-weight mucins requires specialized methods. The present manuscript introduces a slot blotting-based technique to screen post-ultracentrifugation fractions using colorimetric assays. Next, we describe an easy slot blot-based method to characterize mucin-bacteria interactions and to assess the effects of glycan enzymatic treatment on bacterial adhesion.
高分子量粘蛋白的处理需要专门的方法。本手稿介绍了一种基于狭缝印迹的技术，用于使用比色测定法筛选超速离心后的组分。接下来，我们描述一种基于狭缝印迹的简便方法，用于表征粘蛋白与细菌的相互作用，并评估聚糖酶处理对细菌粘附的影响。
