Xiao Xuefeng, Song Yunuo, Hu Jingxuan, Han Jinchen, Xing Wanbin, Liu Chang
Beijing Huijia Private School, Beijing, China.
BASIS International School Shenzhen, Shenzhen, China.
PLoS One. 2025 Jun 11;20(6):e0325926. doi: 10.1371/journal.pone.0325926. eCollection 2025.
With global data expected to reach 175 zettabytes by 2025, traditional storage methods face unprecedented challenges, including security risks, limited durability, and high maintenance costs associated with centralized infrastructure. While DNA-based storage systems have demonstrated high density and chemical stability, most existing methods focus primarily on static storage, lacking effective strategies for secure and controllable information transmission. Coli Bond offers a revolutionary approach by combining the molecular precision of DNA storage with the controllable dynamics of synthetic biology, providing an innovative platform for data encryption and storage. In this system, controllable dynamics refer to information transfer regulated by caffeine concentration and temperature. The system leverages synthetic biology to engineer an auxotrophic Escherichia coli strain with a caffeine degradation pathway, enabling precise control of information transfer through conditional growth. A temperature-sensitive self-destruction mechanism ensures irreversible destruction of stored information under specific conditions, preventing unauthorized access and enhancing data security. Experimental validation demonstrated the system's stability and reliability under various real-world conditions, including survival and function in commercial beverages, during transmission cycles, and under temperature variation. The results confirmed high transmission efficiency during initial contact and a rapid decline in strain viability after multiple transfers, providing an inherent layer of security. By integrating the high density of DNA storage with the dynamic control capabilities of synthetic biology, "Coli Bond" offers a secure and adaptable platform for the storage and transmission of DNA-encoded information, paving the way for future advancements in information storage and transmission technologies.
预计到2025年全球数据将达到175泽字节，传统存储方法面临前所未有的挑战，包括安全风险、耐久性有限以及与集中式基础设施相关的高昂维护成本。虽然基于DNA的存储系统已展现出高密度和化学稳定性，但大多数现有方法主要侧重于静态存储，缺乏安全可控信息传输的有效策略。Coli Bond通过将DNA存储的分子精度与合成生物学的可控动力学相结合，提供了一种革命性的方法，为数据加密和存储提供了一个创新平台。在这个系统中，可控动力学是指由咖啡因浓度和温度调节的信息传递。该系统利用合成生物学设计出一种具有咖啡因降解途径的营养缺陷型大肠杆菌菌株，通过条件生长实现对信息传递的精确控制。一种温度敏感的自我销毁机制确保在特定条件下对存储信息进行不可逆的销毁，防止未经授权的访问并增强数据安全性。实验验证表明该系统在各种实际条件下的稳定性和可靠性，包括在商业饮料中的存活和功能、在传输周期中以及在温度变化下。结果证实了初始接触时的高传输效率以及多次转移后菌株活力的快速下降，提供了一层固有的安全保障。通过将DNA存储的高密度与合成生物学的动态控制能力相结合，“Coli Bond”为DNA编码信息的存储和传输提供了一个安全且适应性强的平台，为信息存储和传输技术的未来发展铺平了道路。
