Soroka Jennifer A, Silva-Campos Matias, Bedon Frank, Dinsdale Adrian, Gleeson Dianne M, Trujillo-González Alejandro
Centre for Conservation Ecology and Genomics, Institute for Applied Ecology, University of Canberra, Canberra, ACT 2617, Australia.
Australian Government Department of Agriculture, Fisheries and Forestry-Plant Innovation Centre Facility, Mickleham, VIC 3064, Australia.
Int J Mol Sci. 2025 Jun 4;26(11):5399. doi: 10.3390/ijms26115399.
Biosecurity is vital to Australia's efforts to prevent and respond to pests and diseases. Here, we report on testing suspected illegal goods (SIGs) as part of an active Australian biosecurity response in Sydney. The Australian Government, Department of Agriculture, Fisheries and Forestry detected and secured consignments containing tuber products of unknown biosecurity risk and origin. Swab samples were collected from vacuum-sealed yam products, organic packing material (background negative controls), and field negative controls to assess possible cross-contamination from the storage facility. DNA from all samples was analysed using high-throughput metabarcoding targeting the Internal Transcribed Spacer 2 (ITS2) and the chloroplast (UAA) P6 Loop gene regions by two independent teams in Australia. A plant community profile comprising Australian native species and other non-native established species would support the notion of produce being harvested and/or packaged domestically, while their absence would suggest foreign production. Of the 5,764,942 total reads produced, the bioinformatic analysis generated 5,181,530 amplicon sequencing variants employed for species identification. Twenty plant taxa were identified via ITS2 and 15 via , corresponding to worldwide distributed plants, non-native species established in Australia, or species not recorded in Australia. No Australian endemic species were detected. The absence of common Australian native plants, combined with the presence of species not known to occur in Australia, provided strong evidence that the suspect tuber products were illegally imported.
生物安全对于澳大利亚预防和应对病虫害的工作至关重要。在此，我们报告了对疑似非法货物（SIGs）进行检测的情况，这是悉尼积极开展的澳大利亚生物安全应对措施的一部分。澳大利亚政府农业、渔业和林业部检测并扣押了含有生物安全风险和来源不明的块茎产品的货物。从真空密封的山药产品、有机包装材料（背景阴性对照）和田间阴性对照中采集拭子样本，以评估储存设施可能存在的交叉污染。澳大利亚的两个独立团队使用针对内部转录间隔区2（ITS2）和叶绿体（UAA）P6环基因区域的高通量宏条形码技术对所有样本的DNA进行了分析。包含澳大利亚本土物种和其他非本土已定居物种的植物群落概况将支持农产品在国内收获和/或包装的观点，而没有这些物种则表明是国外生产。在总共产生的5,764,942条读数中，生物信息学分析生成了5,181,530个用于物种鉴定的扩增子测序变体。通过ITS2鉴定出20个植物分类群，通过[此处原文缺失相关基因区域名称]鉴定出15个，对应于全球分布的植物、在澳大利亚已定居的非本土物种或未在澳大利亚记录的物种。未检测到澳大利亚特有物种。没有常见的澳大利亚本土植物，再加上存在澳大利亚未知的物种，有力地证明了这些可疑块茎产品是非法进口的。
