Laboratory for Core Technology of TCM Quality Improvement and Transformation, School of Pharmaceutical Sciences, The Third Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, P.R. China.
BMC Genomics. 2022 Apr 3;23(1):256. doi: 10.1186/s12864-022-08484-x.
Ophiorrhiza pumila (Rubiaceae) is capable of producing camptothecin (CPT), one monoterpene indole alkaloid extensively employed in the treatment of multiple cancers. Transcription factors (TFs) GATA are a group of transcription regulators involved in plant development and metabolism, and show the feature of binding to the GATA motif within the promoters of target genes. However, GATA TFs have not been characterized in O. pumila.
In this study, a total of 18 GATA genes classified into four subfamilies were identified, which randomly distributed on 11 chromosomes of O. pumila. Synteny analysis of GATA genes between O. pumila and other plant species such as Arabidopsis thaliana, Oryza sativa, Glycine max, Solanum lycopersicum, Vitis vinifera, and Catharanthus roseus genomes were analyzed. Tissue expression pattern revealed that OpGATA1 and OpGATA18 were found to be correlated with ASA, MK, CPR and GPPS, which were highly expressed in leaves. OpGATA7, showed high expression in roots as most of the CPT biosynthetic pathway genes did, suggesting that these OpGATAs may be potential candidates regulating CPT biosynthesis in O. pumila.
In this study, we systematically analyzed the OpGATA TFs, and provided insights into the involvement of OpGATA TFs from O. pumila in CPT biosynthesis.
白花蛇舌草（茜草科）能够产生喜树碱（CPT），一种广泛用于治疗多种癌症的单萜吲哚生物碱。转录因子（TFs）GATA 是一组参与植物发育和代谢的转录调节剂，其特征是结合到靶基因启动子中的 GATA 基序。然而，在白花蛇舌草中尚未对 GATA TF 进行特征描述。
本研究共鉴定出 18 个 GATA 基因，分为四个亚家族，随机分布在白花蛇舌草的 11 条染色体上。对 GATA 基因在白花蛇舌草和其他植物物种（如拟南芥、水稻、大豆、番茄、葡萄和长春花）基因组之间的共线性分析表明，OpGATA1 和 OpGATA18 与 ASA、MK、CPR 和 GPPS 相关，在叶片中高度表达。OpGATA7 在根中表达水平较高，与大多数 CPT 生物合成途径基因相似，表明这些 OpGATAs 可能是调控白花蛇舌草 CPT 生物合成的潜在候选基因。
本研究系统分析了 OpGATA TF，深入探讨了白花蛇舌草 OpGATA TF 参与 CPT 生物合成的机制。
