Borja-Martínez María, Lozano-Sánchez Jesús, Quirantes-Piné Rosa, Almagro Lorena, Pedreño María A, Sabater-Jara Ana B
Department of Plant Biology, Faculty of Biology, University of Murcia, Campus de Espinardo, 30100 Murcia, Spain.
Department of Food Science and Nutrition, University of Granada, Campus of Cartuja, 18071 Granada, Spain.
Plants (Basel). 2025 Jun 3;14(11):1700. doi: 10.3390/plants14111700.
Broccoli agro-industries generate a significant amount of waste, which leads to both environmental and economic problems. These by-products are typically discarded, despite being a valuable source of bioactive compounds, including glucosinolates (GSLs), which can modulate oxidant and inflammatory mediators, exerting anti-inflammatory properties. A crucial challenge in the exploitation of broccoli agro-industrial by-products is the development of sustainable and green extraction technologies. In this work, pressurized liquid extraction (PLE) based on response surface methodology (RSM) has been developed and optimized for the GSLs' extraction, with the aim to evaluate the potential protective mechanisms triggered by GSLs-enriched extracts from broccoli by-products in CCD-18Co myofibroblasts exposed to lipopolysaccharide (LPS). The results obtained showed that the PLE is an efficient and environmentally sustainable alternative procedure for extracting GSLs from broccoli by-products. Furthermore, the GSLs-enriched extract obtained through PLE exhibited antioxidant and anti-inflammatory properties in LPS-stimulated cells, being able to attenuate the expression of some proinflammatory markers (IL-1β, IL-6, IL-8, and TNF-α). Therefore, these compounds could serve as potential nutraceutical agents for the prevention and mitigation of oxidative and inflammatory processes related to intestinal bowel diseases, while also promoting the valorization of these by-products.
西兰花农产品加工业产生大量废弃物，这会导致环境和经济问题。这些副产品通常被丢弃，尽管它们是生物活性化合物的宝贵来源，包括硫代葡萄糖苷（GSLs），其可以调节氧化剂和炎症介质，具有抗炎特性。西兰花农产品加工副产品开发利用中的一个关键挑战是可持续绿色提取技术的发展。在这项工作中，基于响应面法（RSM）开发并优化了加压液体萃取（PLE）用于提取GSLs，目的是评估来自西兰花副产品的富含GSLs的提取物在暴露于脂多糖（LPS）的CCD-18Co成肌纤维细胞中触发的潜在保护机制。获得的结果表明，PLE是从西兰花副产品中提取GSLs的一种高效且环境可持续的替代方法。此外，通过PLE获得的富含GSLs的提取物在LPS刺激的细胞中表现出抗氧化和抗炎特性，能够减弱一些促炎标志物（IL-1β、IL-6、IL-8和TNF-α）的表达。因此，这些化合物可作为潜在的营养剂，用于预防和减轻与肠道疾病相关的氧化和炎症过程，同时还能促进这些副产品的增值利用。
