Zheng Heyu, Zhou Weiping, Mao Meiqi, Wang Yiwei, Tian Xing, Yang Biao, Zhao Lu, Li Shu, Sun Ye, Jiang Zhongjia, Fan Ronghua, Bai Yuxin, Fu Xuanhe, Yang Ke, Liu Guangyan
Molecular Morphology Laboratory, School of Basic Medicine, Shenyang Medical College, Shenyang, 110034, China.
Department of Pathology and Pathophysiology, School of Basic Medicine, Shenyang Medical College, Shenyang, 110034, China.
Biometals. 2025 Jun 14. doi: 10.1007/s10534-025-00703-8.
This study explores the biocompatibility and inhibitory effects of magnesium (Mg) and its alloy, specifically the Mg-Cu alloy, on hepatocellular carcinoma (HCC) cells. The importance of this research stems from the potential use of magnesium alloys as biomaterials in bone repair and tissue engineering, while their effects on cancer cells have not been thoroughly investigated. Existing literature shows that although the degradation properties and biocompatibility of magnesium alloys have been examined, their anticancer properties remain a topic of debate. Thus, this study aims to clarify the impact of the Mg-Cu alloy on HCC cells, providing a theoretical foundation for its use in tumor therapy. We utilized various methods, including sample preparation, cell culture, cell viability assays (CCK8), cell cycle and apoptosis analysis, and luciferase activity detection, to comprehensively evaluate the effects of magnesium and its alloys on cellular behavior. Our findings indicate that the Mg-Cu alloy significantly reduces the viability of HCC cells-Huh7 and enhances apoptosis, with a pronounced effect noted at higher extract concentrations. Additionally, the Mg-Cu alloy effectively inhibits hepatitis B virus (HBV) replication, suggesting its potential as an antiviral agent. In summary, this study highlights the promising anticancer and antiviral properties of the Mg-Cu alloy, indicating its potential applications in biomedical fields. Future research should concentrate on the clinical implications and the mechanisms that underlie these effects.
本研究探讨了镁（Mg）及其合金，特别是Mg-Cu合金对肝癌（HCC）细胞的生物相容性和抑制作用。这项研究的重要性源于镁合金作为生物材料在骨修复和组织工程中的潜在应用，而它们对癌细胞的影响尚未得到充分研究。现有文献表明，尽管已经研究了镁合金的降解特性和生物相容性，但其抗癌特性仍是一个有争议的话题。因此，本研究旨在阐明Mg-Cu合金对HCC细胞的影响，为其在肿瘤治疗中的应用提供理论基础。我们采用了多种方法，包括样品制备、细胞培养、细胞活力测定（CCK8）、细胞周期和凋亡分析以及荧光素酶活性检测，以全面评估镁及其合金对细胞行为的影响。我们的研究结果表明，Mg-Cu合金显著降低了HCC细胞-Huh7的活力并增强了凋亡，在较高提取物浓度下效果显著。此外，Mg-Cu合金有效抑制乙型肝炎病毒（HBV）复制，表明其作为抗病毒剂的潜力。总之，本研究突出了Mg-Cu合金具有前景的抗癌和抗病毒特性，表明其在生物医学领域的潜在应用。未来的研究应集中在这些作用的临床意义和潜在机制上。
