Pavlov A V, Antipanova E M
Arkh Anat Gistol Embriol. 1985 Jul;89(7):70-5.
A quantitative analysis on homogeneity degree in distribution of proliferating parathyrocytes in the parathyroid glands under conditions of an acute stimulation of their function, produced by bilateral nephrectomy (12 rats, 50-70 h after the operation) and by hemiparathyroidectomy (12 rats, 2-3 days after the operation) have been performed. Six intact animals serve as the control. In order to reveal proliferating cells, 1 h before sacrifice 3H-thymidine (1 mc Ci/g) is injected intraperitoneally, and to some animals--colchicine (1 mg/kg) is injected 6 h before the sacrifice. By means of successive investigation of the glands serial sections along their whole area the amount of the microscope field of vision, having various content of proliferating cells are calculated; using the criterion chi 2, the correspondence of the empiric distributions obtained to the Poisson distribution are estimated. In the hyperfunctioning parathyroid glands the proliferating cells form foci of clusters and rarifaction. A similar clustering appears when a definite level of mitotic activity of the parathyrocytes is reached (1-1.2%), probably reflecting certain regional peculiarities of their functional state.
对双侧肾切除（12只大鼠，术后50 - 70小时）和甲状旁腺半切除（12只大鼠，术后2 - 3天）所引起的甲状旁腺功能急性刺激条件下，甲状旁腺中增殖的甲状旁腺细胞分布均匀度进行了定量分析。6只完整动物作为对照。为了显示增殖细胞，在处死前1小时腹腔注射3H - 胸腺嘧啶核苷（1微居里/克），对一些动物在处死前6小时注射秋水仙碱（1毫克/千克）。通过对腺体沿其整个区域的连续切片进行研究，计算具有不同增殖细胞含量的显微镜视野数量；使用卡方检验标准，估计所得经验分布与泊松分布的符合程度。在功能亢进的甲状旁腺中，增殖细胞形成簇状和稀疏区域。当甲状旁腺细胞的有丝分裂活性达到一定水平（1 - 1.2%）时会出现类似的聚集，这可能反映了其功能状态的某些区域特性。
