Kumar A, Sharma K K, Pattabiraman T N
Biochem Med. 1985 Aug;34(1):112-9. doi: 10.1016/0006-2944(85)90068-7.
Glucose incorporated in vitro during nonenzymatic glucosylation into albumin and hemoglobin was fully reducible by sodium borohydride unlike native albumin. Further, a prior hydrolysis under mild conditions (1 M oxalic acid:2 M HCl, 4 hr) was not required for in vitro incorporated glucose to yield maximal color intensity in the phenol-sulfuric acid reaction. Glucosyl-albumin, glucosyl-crystallin, and hemoglobin A1 behaved similarly in this respect. Hexose bound to HbA0 which alone showed an enhanced color intensity on prior acid hydrolysis was also not easily reduced by sodium borohydride. L-Cysteine (0.023 M) enhanced the color yield of glucosyl-hemoglobin, glucosyl-albumin, and glucosyl-crystallin to a lesser extent compared to fructose in the phenol-sulfuric acid reaction. Urea (6 M) also marginally increased the color intensity of glucosyl proteins and fructose.
与天然白蛋白不同，在非酶糖基化过程中体外掺入白蛋白和血红蛋白中的葡萄糖可被硼氢化钠完全还原。此外，体外掺入的葡萄糖在酚-硫酸反应中产生最大颜色强度时，不需要在温和条件下（1M草酸：2M盐酸，4小时）预先水解。在这方面，糖基化白蛋白、糖基化晶状体蛋白和血红蛋白A1表现相似。与单独的HbA0结合的己糖在预先酸水解时仅显示出增强的颜色强度，也不易被硼氢化钠还原。在酚-硫酸反应中，L-半胱氨酸（0.023M）与果糖相比，对糖基化血红蛋白、糖基化白蛋白和糖基化晶状体蛋白的颜色产率增强程度较小。尿素（6M）也略微增加了糖基化蛋白质和果糖的颜色强度。
