Beena Rai K, Krishna Sharma K, Pattabiraman T N
Biochem Med. 1984 Feb;31(1):65-72. doi: 10.1016/0006-2944(84)90060-7.
Hemolysates were treated with HCl (0.18 M)-acetone solution to remove heme and the globin precipitated was washed with acetone. It was dissolved in 1 ml of 0.05 M Tris-HCl, pH 7.0, subjected to heat treatment for 10 min at 100 degrees C to remove traces of acetone, and treated with 0.05 ml of 80% phenol and 3 ml of H2SO4. The color was measured at 480 nm. Glucosylhemoglobin values in control subjects and diabetics were respectively 0.286 +/- 0.051 and 0.513 +/- 0.081 mole hexose/mole hemoglobin. The increase in diabetics was highly significant (P less than 0.001). A good correlation (r = 0.85) between fasting blood sugar values and glucosylhemoglobin level was observed. When globin solution was subjected to 4 hr hydrolysis with HCl-oxalic acid (2 and 1 mole/liter) solution prior to phenol-sulfuric acid reaction, estimated glucosylhemoglobin values increased to 0.720 +/- 0.083 in control subjects and 1.036 +/- 0.115 in diabetics. The possible reasons for this increase are discussed.
溶血产物用0.18M盐酸 - 丙酮溶液处理以去除血红素，沉淀的珠蛋白用丙酮洗涤。将其溶解于1ml 0.05M Tris - HCl（pH 7.0）中，在100℃下热处理10分钟以去除痕量丙酮，并用0.05ml 80％苯酚和3ml硫酸处理。在480nm处测量颜色。对照组和糖尿病患者的糖化血红蛋白值分别为0.286±0.051和0.513±0.081摩尔己糖/摩尔血红蛋白。糖尿病患者的增加非常显著（P小于0.001）。观察到空腹血糖值与糖化血红蛋白水平之间有良好的相关性（r = 0.85）。在进行酚 - 硫酸反应之前，将珠蛋白溶液用HCl - 草酸（2和1摩尔/升）溶液进行4小时水解，对照组的估计糖化血红蛋白值增加到0.720±0.083，糖尿病患者增加到1.036±0.115。讨论了这种增加的可能原因。
