Wang Lijie, Wang Zixuan, Chang Jiarong, Zhou Zhiyue, Wang Xihan, Li Zhaoming, Li Dawei, Wang Fang, Zhang Wen, Gunarathne Kannapathirage Dilini Maheshika, Meng Xin
Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.
Shandong Academy of Pharmaceutical Sciences, Shandong Engineering Research Center of New Sustained and Controlled Release Formulations and Drug Targeted Delivery System, Shandong Key Laboratory of Targeted Drug Delivery and Advanced Pharmaceutics, Shandong Engineering Research Center for Transdermal Drug Delivery Systems, Jinan 250101, China.
ACS Appl Mater Interfaces. 2025 Jul 2;17(26):37679-37697. doi: 10.1021/acsami.5c07412. Epub 2025 Jun 16.
Infected skin wound healing involves a series of dynamic and complex physiological processes. In this study, we reported a dissolvable microneedle (MN) with pullulan as the matrix material. The antimicrobial peptide LL37 was immobilized within ZIF-8 to form hybrid nanoparticle LL37@ZIF-8 (LZ). This nanoparticle was then loaded onto the tip of the microneedle along with Prussian Blue (PB) nanozymes to obtain PB+LZ@MN, which exhibits both antimicrobial and antioxidative activities, for the healing of infected skin wounds. The MN, with good mechanical properties, could rapidly dissolve its tip upon insertion into the skin, releasing LZ and PB into the wound within 3 min. The MN had good physicochemical properties and biocompatibility and was shown to reduce inflammation and stimulate angiogenesis by enhancing collagen deposition and re-epithelialization in the Sprague-Dawley (SD) rat skin defect infection model. On the 10th day of wound healing, the healing rate of the PB+LZ@MN group was as high as 99%, which was significantly higher than that of the control at 84%, significantly accelerating the healing of the infected wound. This multifunctional MN is expected to provide a competitive biomaterial for accelerated infected wound healing.
感染性皮肤伤口愈合涉及一系列动态且复杂的生理过程。在本研究中，我们报道了一种以普鲁兰多糖为基质材料的可溶解微针（MN）。抗菌肽LL37固定于ZIF-8内以形成杂化纳米颗粒LL37@ZIF-8（LZ）。然后将该纳米颗粒与普鲁士蓝（PB）纳米酶一起加载到微针尖端，以获得具有抗菌和抗氧化活性的PB+LZ@MN，用于感染性皮肤伤口的愈合。该微针具有良好的机械性能，插入皮肤后其尖端能迅速溶解，在3分钟内将LZ和PB释放到伤口中。该微针具有良好的物理化学性质和生物相容性，在Sprague-Dawley（SD）大鼠皮肤缺损感染模型中，通过增强胶原蛋白沉积和再上皮化，显示出可减轻炎症并刺激血管生成。在伤口愈合的第10天，PB+LZ@MN组的愈合率高达99%，显著高于对照组的84%，明显加速了感染伤口的愈合。这种多功能微针有望为加速感染伤口愈合提供一种有竞争力的生物材料。
