Xin Xin, Li Jiaxi, Zhang Jinfu, Wu Haicui
Guanghua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Brain Behav. 2025 Jun;15(6):e70627. doi: 10.1002/brb3.70627.
Inflammation has been implicated in both reproduction and sleep; however, the relationships remain unclear, and conclusive evidence for genetic associations is lacking.
This study utilized the 2013-2020 National Health and Nutrition Examination Survey (NHANES) data and conducted a Mendelian randomization (MR) analysis to investigate the associations among inflammation, sleep, and female infertility. A cross-sectional study was performed on 370 infertile women aged 20-44 years. Multiple linear regression was applied to evaluate the associations between sleep and the inflammatory markers. Subgroup and interaction analyses were conducted to assess the robustness of the findings. MR analysis was performed on 91 circulating inflammatory proteins, 11 sleep traits, and infertility to examine potential genetic associations.
A total of 370 infertile participants aged 20-44 years were included. The logarithmic platelet-lymphocyte ratio (PLR) was significantly higher in the sleep disorder group (p < 0.05). After adjusting for potential confounders, sleep disorders remained associated with a reduction in PLR (β = -0.145, 95% CI: -0.267 to -0.023, p < 0.05). MR analysis using the inverse variance weighted (IVW) method indicated that insomnia, subjective long sleep duration, and high sleep efficiency were associated with increased levels of oncostatin-M, artemisinin, and adenosine deaminase, all of which are implicated in infertility. Additionally, the morning type was associated with increased levels of C-X-C motif chemokine 5, which reduced the risk of infertility.
Sleep is associated with various inflammatory factors in the body and may contribute to infertility. Inflammation appears to play a key role in mediating the complex interplay between sleep and reproduction. These findings highlight the potential value of screening and managing specific inflammatory markers in infertile patients with sleep disorders to improve reproductive outcomes. However, further clinical studies and mechanistic experiments are needed to validate the genetic associations identified in this study.
炎症与生殖和睡眠均有关联；然而，它们之间的关系仍不明确，且缺乏基因关联的确凿证据。
本研究利用2013 - 2020年国家健康与营养检查调查（NHANES）数据，进行孟德尔随机化（MR）分析，以探究炎症、睡眠和女性不孕症之间的关联。对370名年龄在20 - 44岁的不孕女性进行了横断面研究。应用多元线性回归评估睡眠与炎症标志物之间的关联。进行亚组和交互分析以评估研究结果的稳健性。对91种循环炎症蛋白、11种睡眠特征和不孕症进行了MR分析，以检查潜在的基因关联。
共纳入370名年龄在20 - 44岁的不孕参与者。睡眠障碍组的对数血小板 - 淋巴细胞比率（PLR）显著更高（p < 0.05）。在调整潜在混杂因素后，睡眠障碍仍与PLR降低相关（β = -0.145，95% CI：-0.267至 -0.023，p < 0.05）。使用逆方差加权（IVW）方法的MR分析表明，失眠、主观睡眠时间长和睡眠效率高与抑瘤素 - M、青蒿素和腺苷脱氨酶水平升高相关，所有这些都与不孕症有关。此外，晨型与C - X - C基序趋化因子水平升高相关，这降低了不孕风险。
睡眠与体内多种炎症因子相关，可能导致不孕。炎症似乎在介导睡眠与生殖之间的复杂相互作用中起关键作用。这些发现凸显了在患有睡眠障碍的不孕患者中筛查和管理特定炎症标志物以改善生殖结局的潜在价值。然而，需要进一步的临床研究和机制实验来验证本研究中确定的基因关联。
