通过一维和二维核磁共振光谱对阿片肽德莫啡肽进行的构象研究。
A conformational study of the opioid peptide dermorphin by one-dimensional and two-dimensional nuclear magnetic resonance spectroscopy.
作者信息
Pastore A, Temussi P A, Salvadori S, Tomatis R, Mascagni P
出版信息
Biophys J. 1985 Aug;48(2):195-200. doi: 10.1016/S0006-3495(85)83772-3.
Abstract
Dermorphin, a natural peptide opioid containing a D-Ala2 residue, has been studied in dimethyl sulfoxide (DMSO) solution by means of several one-dimensional and two-dimensional 1H nuclear magnetic resonance (NMR) methods at various fields from 80 to 600 MHz. The combined use of conventional NMR parameters and of nuclear Overhauser effect effects points to an essentially extended structure. This conformation may be, in part, the result of strong interaction of the amide groups with DMSO molecules.
摘要
皮啡肽是一种含有D - Ala2残基的天然肽类阿片样物质，已在二甲基亚砜（DMSO）溶液中，通过多种一维和二维1H核磁共振（NMR）方法，在80至600 MHz的不同磁场下进行了研究。常规NMR参数与核Overhauser效应的联合使用表明其结构基本呈伸展状。这种构象可能部分是酰胺基团与DMSO分子强烈相互作用的结果。
相似文献
1
A conformational study of the opioid peptide dermorphin by one-dimensional and two-dimensional nuclear magnetic resonance spectroscopy.通过一维和二维核磁共振光谱对阿片肽德莫啡肽进行的构象研究。
Biophys J. 1985 Aug;48(2):195-200. doi: 10.1016/S0006-3495(85)83772-3.
2
Proton magnetic resonance studies on dermorphin and its peptide fragments.关于皮啡肽及其肽片段的质子磁共振研究。
Biopolymers. 1984 Nov;23(11 Pt 1):2349-60. doi: 10.1002/bip.360231116.
3
Mono and two-dimensional 500-MHz characterization of synthetic bombesin and related peptides in DMSO and DMSO-water.在二甲基亚砜和二甲基亚砜 - 水体系中对合成铃蟾肽及相关肽进行一维和二维500兆赫兹表征。
Biopolymers. 1989 Jan;28(1):421-40. doi: 10.1002/bip.360280139.
4
NMR studies of a series of dehydrodermorphins.一系列脱氢德莫啡的核磁共振研究。
Biopolymers. 1989 Jan;28(1):129-38. doi: 10.1002/bip.360280115.
5
A conformational comparison of two stereoisomeric cyclic dermorphin analogues employing NMR and computer simulations.
Biopolymers. 1990 May-Jun;29(6-7):943-52. doi: 10.1002/bip.360290607.
6
Dermorphin tetra- and heptapeptide analogues containing a [3,4] amide bond replacement by a carbon-carbon double and single bond.含有碳 - 碳双键和单键取代[3,4]酰胺键的 Dermorphin 四肽和七肽类似物。
Int J Pept Protein Res. 1992 Apr;39(4):315-21. doi: 10.1111/j.1399-3011.1992.tb01591.x.
7
A 500 MHz study of peptide T in a DMSO solution.在二甲基亚砜溶液中对肽 T 的 500 兆赫研究。
FEBS Lett. 1988 Apr 11;231(1):159-63. doi: 10.1016/0014-5793(88)80723-3.
8
Synthesis, conformation and opioid activity of deltorphins.强啡肽的合成、构象与阿片样活性
Biochem Biophys Res Commun. 1995 Feb 6;207(1):99-104. doi: 10.1006/bbrc.1995.1158.
9
Dermorphin interaction with rat brain opioid receptors: involvement of hydrophobic sites in the binding domain.强啡肽与大鼠脑阿片受体的相互作用：结合域中疏水位点的作用。
Mol Pharmacol. 1990 Jun;37(6):886-92.
10
[Study of bradykinin conformation in a dimethylsulfoxide solution by two-dimensional 1H-NMR spectroscopy].[利用二维¹H-NMR光谱法研究缓激肽在二甲基亚砜溶液中的构象]
Bioorg Khim. 1986 Apr;12(4):437-47.
本文引用的文献
1
Synthesis of dermorphins, a new class of opiate-like peptides.新型阿片样肽类物质——皮啡肽的合成
Int J Pept Protein Res. 1981 Feb;17(2):263-72. doi: 10.1111/j.1399-3011.1981.tb01991.x.
2
Synthesis and pharmacological activity of dermorphin and its N-terminal sequences.德尔吗啡及其N端序列的合成与药理活性
Int J Pept Protein Res. 1982 May;19(5):536-42. doi: 10.1111/j.1399-3011.1982.tb02639.x.
3
Proton magnetic resonance studies on dermorphin and its peptide fragments.关于皮啡肽及其肽片段的质子磁共振研究。
Biopolymers. 1984 Nov;23(11 Pt 1):2349-60. doi: 10.1002/bip.360231116.
4
Nuclear Overhauser effect and cross-relaxation rate determinations of dihedral and transannular interproton distances in the decapeptide tyrocidine A.十肽短杆菌酪肽A中二面角和跨环质子间距离的核Overhauser效应及交叉弛豫率测定
Biophys J. 1980 Nov;32(2):807-36. doi: 10.1016/S0006-3495(80)85018-1.
5
Characterization of opioid receptors in nervous tissue.神经组织中阿片受体的特性研究。
Proc R Soc Lond B Biol Sci. 1980 Oct 29;210(1178):113-22. doi: 10.1098/rspb.1980.0122.
6
254. The solution conformation of cyclo-glycyl-L-prolyl-glycyl-glycyl-L-prolyl-glycyl. Communication 22 (preliminary) on homodetic cyclic polypeptides.环甘氨酰-L-脯氨酰-甘氨酰-甘氨酰-L-脯氨酰-甘氨酰的溶液构象。关于同系环多肽的通讯22（初步报告）
Helv Chim Acta. 1972;55(7):2545-9. doi: 10.1002/hlca.19720550731.
7
Intramolecular 1H nuclear Overhauser effect study of the solution conformation of valinomycin in dimethyl sulfoxide.在二甲基亚砜中缬氨霉素溶液构象的分子内1H核Overhauser效应研究
Biochemistry. 1976 Dec 28;15(26):5721-9. doi: 10.1021/bi00671a007.
8
The quantitation of nuclear Overhauser effect methods for total conformational analysis of peptides in solution. Application to gramicidin S.溶液中肽段全构象分析的核Overhauser效应方法定量。应用于短杆菌肽S。
Biophys J. 1978 Dec;24(3):815-32. doi: 10.1016/S0006-3495(78)85422-8.
9
Individual residue correlation times of peptides from proton relaxation parameters: application to gramicidin S.
Biochem Biophys Res Commun. 1978 Aug 29;83(4):1380-7. doi: 10.1016/0006-291x(78)91374-8.
Zotero 插件