溶液中肽段全构象分析的核Overhauser效应方法定量。应用于短杆菌肽S。
The quantitation of nuclear Overhauser effect methods for total conformational analysis of peptides in solution. Application to gramicidin S.
作者信息
Jones C R, Sikakana C T, Hehir S, Kuo M C, Gibbons W A
出版信息
Biophys J. 1978 Dec;24(3):815-32. doi: 10.1016/S0006-3495(78)85422-8.
Abstract
The [1H:1H] nuclear Overhauser effects (NOE's) and spin-lattice relaxation times (T1's) are reported for the backbone protons of the decapeptide gramicidin S. Several methods for calculating interproton distances from these measurements are presented. Ratios of interproton distances were obtained from [1H:1H] NOE's and from the combination of [1H:1H]NOE'S and T1 values. Actual proton-proton distances were calculated from these ratios either by using the known distance between two geminal protons or distances derived from scalar coupling constants. The interproton distances calculated for gramicidin S are consistent with a II' beta-turn/antiparallel beta-sheet conformation.
摘要
报道了短杆菌肽S十肽主链质子的[1H:1H]核Overhauser效应（NOE）和自旋晶格弛豫时间（T1）。介绍了几种从这些测量值计算质子间距离的方法。质子间距离的比值由[1H:1H]NOE以及[1H:1H]NOE和T1值的组合得到。通过使用两个偕质子之间的已知距离或由标量耦合常数得出的距离，从这些比值计算出实际的质子-质子距离。为短杆菌肽S计算出的质子间距离与II'β-转角/反平行β-折叠构象一致。
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