Lenkei R, Ghetie V
J Immunol Methods. 1977;16(1):23-30. doi: 10.1016/0022-1759(77)90036-9.
Three methods for detection and quantitation of anti-albumin autoantibodies (AAA) in human sera have been developed based on their reaction with a new antigenic site of glutaraldehyde-polymerized human serum albumin (HSA). The first is a double diffusion technique (ID-HSA) were the sera to be tested are reacted with a copolymer of glutaraldehyde activated HSA (copoly-HSA). The reaction is estimated by the lowest copoly HSA concentration giving precipitation with the undiluted sera. The second method is based on agglutination (HA) by AAA positive sera of human red blood cells coated with glutaraldehyde-polymerized HSA (EPA). The reaction is quantified as the highest dilution of sera giving agglutination. The third method uses glutaraldehyde insolubilized HSA as an immunoadorbent able to bind AAA in positive sera. The quantity of protein adsorbed, expessed in microng antibody/ml serum, measures the level of AAA in sera. Significant correlation between the values of AAA as determined by all three methods presented above were recorded.
基于抗白蛋白自身抗体（AAA）与人血清中戊二醛聚合人血清白蛋白（HSA）新抗原位点的反应，已开发出三种检测和定量人血清中抗白蛋白自身抗体的方法。第一种是双向扩散技术（ID-HSA），即将待测血清与戊二醛活化的HSA共聚物（copoly-HSA）反应。通过能与未稀释血清产生沉淀的最低共聚物HSA浓度来评估反应。第二种方法基于用戊二醛聚合HSA（EPA）包被的人红细胞被AAA阳性血清凝集（HA）。反应定量为产生凝集的血清最高稀释度。第三种方法使用戊二醛不溶性HSA作为免疫吸附剂，能够结合阳性血清中的AAA。吸附的蛋白量以微克抗体/毫升血清表示，可测定血清中AAA的水平。记录了通过上述所有三种方法测定的AAA值之间的显著相关性。
