Jeong Yoonsoo, Yun Taesik, Kim Hakhyun, Koo Yoonhoi, Kang Ji-Houn, Yang Mhan-Pyo, Kang Byeong-Teck
Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea.
Yoonsoo Jeong and Taesik Yun contributed equally to this work.
Vet Med (Praha). 2021 Oct 31;66(10):413-422. doi: 10.17221/58/2020-VETMED. eCollection 2021 Oct.
This study was performed to induce atopic dermatitis (AD) using nongenetically predisposed Beagle dogs. Five healthy Beagle dogs were used. Twice weekly for 12 weeks, the dogs were painted on the axillae and groin with a solution of (). Each dog was thereafter placed in a cage where a house dust mite (HDM) solution was applied on the bottom of the cage. The dog remained in the cage for 3 h daily for 3 consecutive days for the environmental exposure to HDM. Serum samples were collected at 0 week and 6 weeks after sensitisation, and at 0 h and 72 h after the environmental exposure. During the environmental exposure, skin biopsies were obtained at 0 h, 36 h, and 72 hours. After the first environmental exposure, no dog had any marked clinical sign. An additional sensitisation was subsequently administered for 10-13 weeks. Three of the five dogs developed pruritic dermatitis with skin lesions after the second exposure. The histopathology of the lesions revealed severe infiltration of inflammatory cells and dermal oedema. The levels of -specific IgE were also elevated. This study demonstrated that AD could be induced by epicutaneous sensitisation with HDM in nongenetically predisposed dogs.
本研究旨在使用非遗传易感性的比格犬诱导特应性皮炎（AD）。使用了5只健康的比格犬。每周两次，持续12周，在犬的腋窝和腹股沟涂抹（）溶液。此后，将每只犬置于一个笼子中，在笼子底部涂抹屋尘螨（HDM）溶液。犬连续3天每天在笼子里待3小时，以使其环境暴露于HDM。在致敏后0周和6周以及环境暴露后0小时和72小时采集血清样本。在环境暴露期间，于0小时、36小时和72小时获取皮肤活检样本。首次环境暴露后，没有犬出现任何明显的临床症状。随后进行了为期10 - 13周的额外致敏。5只犬中有3只在第二次暴露后出现了伴有皮肤病变的瘙痒性皮炎。病变的组织病理学显示有严重的炎症细胞浸润和真皮水肿。特异性IgE水平也升高。本研究表明，在非遗传易感性犬中，HDM经皮致敏可诱导AD。
