: The safety profile of low-carbohydrate diets is controversial and poorly understood. We investigated the effects of low-moderate carbohydrate intake on dietary acid load (DAL), an emerging health risk factor and novel clinical marker associated with numerous adverse clinical outcomes. : We used data from the National Health and Nutrition Examination Surveys to investigate how low-moderate carbohydrate intake quantitatively affects DAL (as assessed by PRAL and NEAP scores) and to contrast the results to DAL scores in individuals that meet carbohydrate intake recommendations. : We analyzed data from 23 825 individuals, of which 4891 consumed a low-moderate carbohydrate diet. Said individuals derived 37.84% of energy from carbohydrates, tended to be male, had a mean BMI of 28.47 kg/m, and consumed significantly more energy from fat and protein (≤.01 for both) than individuals that met carbohydrate recommendations Low-moderate carbohydrate intake was associated with a significantly higher DAL. Mean PRAL, NEAP, and NEAP values were 26.12, 71.02, and 68.98 mEq/d, respectively. Multivariate regression revealed the highest DAL scores in individuals on a low-carbohydrate diet, obtaining <26% of energy from carbohydrates. : Low-moderate carbohydrate intake is associated with increased DAL scores, which has been repeatedly associated with various health repercussions.