Zhao Yunbo, Yang Fangyuan, Zhao Lefa, Xu Guangda
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Department of Scientific Socialism (Political Science), Party School of the CPC Liaoning Provincial Committee, Shenyang, 110000, China.
J Fluoresc. 2025 Jun 23. doi: 10.1007/s10895-025-04413-9.
In this study, molybdenum disulfide quantum dots (MoS QDs) were synthesized via a one-step hydrothermal method, and a simple, rapid fluorescent sensing platform for dopamine (DA) detection was established based on fluorescence quenching. Experimental results demonstrated that DA is oxidized to dopamine quinone under alkaline conditions, leading to fluorescence quenching of MoS QDs through the synergistic effects of inner filter effect (IFE) and electron transfer (ET). The optimized method exhibited a linear response at 434 nm (200-600 µM, R = 0.9980) with a detection limit of 4 µM. The sensor showed high selectivity for DA and was successfully applied to spiked recovery tests in human urine samples, achieving recoveries of 95-104.6%. The stability of MoS QDs, simplicity of preparation, and practical applicability highlight its potential for DA analysis in complex biological samples.
在本研究中，通过一步水热法合成了二硫化钼量子点（MoS QDs），并基于荧光猝灭建立了一种简单、快速的多巴胺（DA）检测荧光传感平台。实验结果表明，在碱性条件下，DA被氧化为多巴胺醌，通过内滤效应（IFE）和电子转移（ET）的协同作用导致MoS QDs的荧光猝灭。优化后的方法在434 nm处呈现线性响应（200 - 600 µM，R = 0.9980），检测限为4 µM。该传感器对DA具有高选择性，并成功应用于人尿样的加标回收试验，回收率为95 - 104.6%。MoS QDs的稳定性、制备的简易性和实际适用性突出了其在复杂生物样品中进行DA分析的潜力。
