Wang Jun, Zhang Jia-Xin, Dai Xiao-Feng, Guo Qi-Tang, Chen Jie-Yin, Li Ying, Zhang Dan-Dan
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Fujian Agriculture and Forestry University, Fuzhou 350002, China.
J Agric Food Chem. 2025 Jul 2;73(26):16109-16120. doi: 10.1021/acs.jafc.5c00921. Epub 2025 Jun 23.
The necrotrophic phytopathogen causes leaf spot and root rot in tobacco, which significantly reduce the yield and quality of the crop. Recently, much attention has been paid to effectors, but only a few of them have been characterized. Here, we identified a CAP domain-containing protein, RsCAP3, with a functional signal peptide, which triggers slight cell death on tobacco leaves. Meanwhile, RsCAP3 dramatically induces salicylic acid (SA) signaling but inhibits jasmonic acid (JA) signaling, increasing the plant's susceptibility to . Moreover, we demonstrated that RsCAP3 interacts with Nb14-3-3b, a negative regulator of tobacco resistance to . Correspondingly, we also found that transient expression of activates SA signaling while suppressing JA signaling. Based on these results, we propose that RsCAP3 hijacks Nb14-3-3b to disrupt JA and SA pathways, thereby facilitating the infection of . Our findings highlight a novel virulence strategy in which interferes with the JA-/SA-mediated defense response.
这种坏死性植物病原菌会导致烟草叶斑病和根腐病，显著降低作物的产量和品质。最近，效应子受到了广泛关注，但其中只有少数被鉴定出来。在这里，我们鉴定出一种含有CAP结构域的蛋白RsCAP3，它带有一个功能性信号肽，能在烟草叶片上引发轻微的细胞死亡。同时，RsCAP3显著诱导水杨酸（SA）信号传导，但抑制茉莉酸（JA）信号传导，增加了植物对[病原菌名称未给出]的易感性。此外，我们证明RsCAP3与Nb14-3-3b相互作用，Nb14-3-3b是烟草对[病原菌名称未给出]抗性的负调控因子。相应地，我们还发现[基因名称未给出]的瞬时表达激活SA信号传导，同时抑制JA信号传导。基于这些结果，我们提出RsCAP3劫持Nb14-3-3b来破坏JA和SA途径，从而促进[病原菌名称未给出]的感染。我们的发现突出了一种新的致病策略，即[病原菌名称未给出]干扰JA/SA介导的防御反应。
