Montealegre F, Levy M G, Ristic M, James M A
Infect Immun. 1985 Nov;50(2):523-6. doi: 10.1128/iai.50.2.523-526.1985.
Bovine blood mononuclear phagocytes from babesia-free cattle were cultured in vitro. Cell monolayers were treated with culture-derived soluble Babesia bovis exoantigens, immune complexes, and bovine anti-B. bovis immune serum. Subsequently, the monolayers were washed free of the reagents and allowed to develop further in the presence of standard culture medium. Transfer of supernatant media from these cultures to those of B. bovis revealed the presence of growth-inhibiting factors. These factors were thermostable, nondialyzable, and were degraded by freeze-thawing, and their action was concentration dependent. Supernatants from antigen- and immune complex-treated monolayers demonstrated greater inhibitory effects than did supernatants from antibody-treated or untreated monolayers. Erythrocytes incubated with supernatant medium from antigen-treated monolayers did not support growth of B. bovis as well as did erythrocytes incubated with supernatants from untreated monocyte monolayers. This result suggests that the mechanism of action of soluble factors could be through some modification of the erythrocyte such as the blockage of active transport of essential nutrients.
从无巴贝斯虫的牛身上获取牛血单核吞噬细胞并进行体外培养。细胞单层用培养产生的可溶性牛巴贝斯虫外抗原、免疫复合物和牛抗牛巴贝斯虫免疫血清处理。随后，将单层细胞洗涤以去除试剂，并在标准培养基存在下继续培养。将这些培养物的上清培养基转移至牛巴贝斯虫培养物中，发现存在生长抑制因子。这些因子耐热、不可透析，且经冻融后会降解，其作用呈浓度依赖性。抗原和免疫复合物处理的单层细胞的上清液比抗体处理或未处理的单层细胞的上清液显示出更大的抑制作用。与抗原处理的单层细胞的上清培养基一起孵育的红细胞，不如与未处理的单核细胞单层细胞的上清液一起孵育的红细胞那样支持牛巴贝斯虫的生长。这一结果表明，可溶性因子的作用机制可能是通过对红细胞的某种修饰，例如阻断必需营养物质的主动转运。
