Ikeda Shin-Ichi, Lee Deokho, Chen Junhan, Fukuda Shinji, Negishi Kazuno, Tsubota Kazuo, Kurihara Toshihide
Laboratory of Photobiology, Keio University School of Medicine, Shinjuku-ku, Tokyo, Japan.
Department of Ophthalmology, Keio University School of Medicine, Shinjuku-ku, Tokyo, Japan.
Invest Ophthalmol Vis Sci. 2025 Jun 2;66(6):76. doi: 10.1167/iovs.66.6.76.
The prevalence of myopia has increased worldwide in recent decades, shifting the focus in research from genetic to environmental factors. The roles of diet in the development of myopia may be directly associated with gut microbiota composition. Therefore this study evaluated the effects of antibiotic-induced gut dysbiosis on the development of negative lens-induced myopia.
We administered several antibiotics (ampicillin, vancomycin, neomycin, or a mixture) to induce gut dysbiosis in male C57BL/6J mice with negative lens-induced myopia. Gut microbiome profiles were analyzed by 16 S rRNA gene sequencing.
Mice administered vancomycin, neomycin, and a mixture of three antibiotics exhibited resistance to lens-induced myopia, unlike control or ampicillin-administered mice. Further analyses revealed no specific trend in the gut microbiota composition and diversity related to myopia resistance, except for an increase in the abundance of Clostridiaceae.
These findings demonstrate the potential role of the gut microbiome, particularly Clostridiaceae family, in myopia susceptibility. This study offers new insights into the preventive strategies and therapeutic interventions to mitigate myopia development.
近几十年来，近视在全球范围内的患病率有所上升，这使得研究重点从遗传因素转向环境因素。饮食在近视发展中的作用可能与肠道微生物群组成直接相关。因此，本研究评估了抗生素诱导的肠道菌群失调对负透镜诱导近视发展的影响。
我们对患有负透镜诱导近视的雄性C57BL/6J小鼠施用了几种抗生素（氨苄青霉素、万古霉素、新霉素或混合物）以诱导肠道菌群失调。通过16S rRNA基因测序分析肠道微生物组谱。
与对照或施用氨苄青霉素的小鼠不同，施用万古霉素、新霉素和三种抗生素混合物的小鼠对透镜诱导的近视具有抗性。进一步分析显示，除了梭菌科丰度增加外，肠道微生物群组成和多样性与近视抗性没有特定趋势。
这些发现证明了肠道微生物群，特别是梭菌科，在近视易感性中的潜在作用。本研究为减轻近视发展的预防策略和治疗干预提供了新的见解。
