Chu Hanyu, Zhou Lijie, Mao Yanzhen, Liu Ren, Han Jiaojiao, Su Xiurong, Zhou Jun
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, School of Marine Science, Ningbo University, Ningbo 315832, China.
BioTech (Basel). 2025 Jun 7;14(2):47. doi: 10.3390/biotech14020047.
() is a safe probiotic with no side effects, providing benefits such as gut microbiota regulation and immune enhancement, making it highly valuable with strong potential. However, strains from different sources have unique traits, and whole-genome sequencing (WGS) helps analyse these differences. In this study, we used WGS to examine strains from mice with fish oil-treated smoking-induced pneumonia to better understand their biological functions and explore possible anti-inflammatory mechanisms.
We isolated a strain, CP-1 ( CP-1), from mice intestines where fish oil alleviated smoking-induced pneumonia. Identification of probiotic-related genes by WGS and characterised the strain's probiotic properties.
CP-1 has a single circular chromosome (2,989,570 bp, 46.76% GC content) and no plasmids. COG, GO, and KEGG databases revealed genes linked to carbohydrate metabolism. The CAZy database identified GH25 lysozyme and PL8 polysaccharide lyase genes. KEGG highlighted an antimicrobial peptide ABC transporter permease, while TCDB noted the ABC-type antimicrobial peptide transporter (the main active transport component). KEGG also showed 10 genes for terpenoid skeleton biosynthesis and 5 for keto-glycan unit biosynthesis. Additionally, CP-1 carries metabolic regulators and bacteriocin-related genes.
Whole-genome sequencing analysis revealed that CP-1 has carbohydrate utilisation and potential anti-inflammatory effects at the molecular level. Potential functional genes include carbohydrate transport and hydrolase, antimicrobial peptide ABC transporter and its osmotic enzyme components, bacteriocin immune protein, terpenoid skeleton, and keto-glycan synthesis.
（）是一种安全的益生菌，无副作用，具有调节肠道微生物群和增强免疫力等益处，具有很高的价值和强大的潜力。然而，不同来源的菌株具有独特的特性，全基因组测序（WGS）有助于分析这些差异。在本研究中，我们使用WGS检查了鱼油治疗吸烟诱导性肺炎小鼠的菌株，以更好地了解其生物学功能并探索可能的抗炎机制。
我们从小鼠肠道中分离出一株CP-1（CP-1），其中鱼油缓解了吸烟诱导的肺炎。通过WGS鉴定益生菌相关基因并表征该菌株的益生菌特性。
CP-1有一条单一的环状染色体（2,989,570 bp，GC含量46.76%）且无质粒。COG、GO和KEGG数据库揭示了与碳水化合物代谢相关的基因。CAZy数据库鉴定出GH25溶菌酶和PL8多糖裂解酶基因。KEGG突出显示了一种抗菌肽ABC转运通透酶，而TCDB指出了ABC型抗菌肽转运体（主要的主动转运成分）。KEGG还显示了10个萜类骨架生物合成基因和5个酮聚糖单元生物合成基因。此外，CP-1携带代谢调节因子和细菌素相关基因。
全基因组测序分析表明，CP-1在分子水平上具有碳水化合物利用和潜在的抗炎作用。潜在的功能基因包括碳水化合物转运和水解酶、抗菌肽ABC转运体及其渗透酶成分、细菌素免疫蛋白、萜类骨架和酮聚糖合成。
