Lai Chun-Liang, Mukundan Arvind, Karmakar Riya, Kaur Roopmeet, Huang Kuo-Liang, Wang Hsiang-Chen
Division of Pulmonology and Critical Care, Department of Internal Medicine, Dalin Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, No. 2, Minsheng Road, Dalin, Chiayi City 62247, Taiwan.
School of Medicine, Tzu Chi University, 701 Zhongyang Road, Sec. 3, Hualien 97004, Taiwan.
Biosensors (Basel). 2025 Jun 10;15(6):371. doi: 10.3390/bios15060371.
The growing demand for low-cost biosensors has stimulated the study of new technologies and materials like molybdenum disulfide (MoS). Due to its electroconductive nature and high surface-to-volume ratio, it allows for the ultra-sensitive detection of biomarkers. The crystal structure of MoS provides it with a unique micrometer thickness, making it appropriate for biosensing in healthcare, environmental monitoring, and food safety. As compared to traditional materials, MoS can work without labels (through field-effect transduction or plasmonic shifts) while maintaining biocompatibility and low-cost fabrication, which fill significant voids in the early diagnosis of diseases. This paper provides an overview of the recent advancements in MoS-based biosensors, which are primarily focused on the field-effect transistors and surface plasmon resonance techniques and fabrication methods for MoS-based biosensors like mechanical exfoliation, liquid-phase exfoliation, physical vapor deposition, chemical vapor deposition, and chemical exfoliation, applications in various industries, and their characterization techniques to evaluate the quality and functionality of MoS nanosheets in biosensors. While certain challenges remain like improving conductivity and scalability, MoS-based biosensors serve as a powerful tool for the precise and reliable detection of biomarkers in environmental, food, and healthcare industries.
对低成本生物传感器日益增长的需求刺激了对新技术和材料（如二硫化钼（MoS））的研究。由于其导电性质和高表面积与体积比，它能够实现对生物标志物的超灵敏检测。MoS的晶体结构赋予其独特的微米级厚度，使其适用于医疗保健、环境监测和食品安全领域的生物传感。与传统材料相比，MoS无需标记（通过场效应转导或等离子体激元位移）即可工作，同时保持生物相容性和低成本制造，这填补了疾病早期诊断中的重大空白。本文概述了基于MoS的生物传感器的最新进展，主要聚焦于场效应晶体管和表面等离子体共振技术以及基于MoS的生物传感器的制造方法，如机械剥离、液相剥离、物理气相沉积、化学气相沉积和化学剥离，其在各个行业的应用，以及用于评估生物传感器中MoS纳米片质量和功能的表征技术。尽管仍存在一些挑战，如提高导电性和可扩展性，但基于MoS的生物传感器是环境、食品和医疗保健行业中精确可靠地检测生物标志物的有力工具。
