Gao Hao, Luo Yingjie, Li Qiming, Guo Jiaxuan, Wang Bin
Anhui Provincial Key Laboratory of Biological Control, Anhui Agricultural University, Hefei 230036, China.
Key Laboratory of Pine Wood Nematode Disease Prevention and Control, State Forestry and Grassland Administration, Hefei 230031, China.
J Fungi (Basel). 2025 May 27;11(6):412. doi: 10.3390/jof11060412.
, a well-known species of entomopathogenic fungi with great potential as a biological control agent, is vulnerable to UV damage, which restricts its use in the field. To improve the fungal resistance to UV irradiation, UV-induced mutant strains of s. l. were screened and compared with the wild-type (WT) strain for heat resistance, growth rate, conidial yield, and virulence. Comparative transcriptomic analysis between the selected UV-resistant mutants and the WT was carried out. The results showed that the five mutants exhibited significantly higher heat resistance and growth rates, while the conidial production remained unchanged. Among them, the mutant MaUV-22 exhibited enhanced tolerance to heat, oxidative, osmotic, and SDS stresses as well as increased virulence against . Moreover, the transcriptome analysis of MaUV-22 revealed that the expression of genes associated with the heat shock protein pathway, glutathione S-transferase, and thioredoxin reductase were increased dramatically, while the expression of genes related to the catalase and superoxide dismutase pathways was downregulated. The UV-induction technique is an effective strategy to improve fungal resistance to environmental stresses and affords some other beneficial traits such as better control efficacy of entomopathogenic fungi against pests in the field.
作为一种具有巨大生物防治潜力的著名昆虫病原真菌，易受紫外线损伤，这限制了其在田间的应用。为提高该真菌对紫外线照射的抗性，筛选了紫外线诱导的球孢白僵菌突变菌株，并与野生型（WT）菌株进行耐热性、生长速率、分生孢子产量和毒力的比较。对所选抗紫外线突变体和WT进行了比较转录组分析。结果表明，五个突变体表现出显著更高的耐热性和生长速率，而分生孢子产量保持不变。其中，突变体MaUV - 22对热、氧化、渗透和SDS胁迫的耐受性增强，对球孢白僵菌的毒力增加。此外，MaUV - 22的转录组分析表明，与热休克蛋白途径、谷胱甘肽S -转移酶和硫氧还蛋白还原酶相关的基因表达显著增加，而与过氧化氢酶和超氧化物歧化酶途径相关的基因表达下调。紫外线诱导技术是提高真菌对环境胁迫抗性的有效策略，并赋予一些其他有益特性，如昆虫病原真菌在田间对害虫更好的防治效果。
