Department of Biology, Carleton University, Ottawa, ON, Canada.
BMC Res Notes. 2021 Aug 28;14(1):335. doi: 10.1186/s13104-021-05752-z.
We previously identified propionic acid as a microbially-produced volatile organic compound with fungicidal activity against several pathogenic fungi. The purpose of this work is to better understand how propionic acid affects fungi by examining some of the effects of this compound on the yeast cell.
We show that propionic acid causes a dramatic increase in the uptake of lucifer yellow in yeast cells, which is consistent with enhanced endocytosis. Additionally, using a propidium iodide assay, we show that propionic acid treatment causes a significant increase in the proportion of yeast cells in G and a significant decrease in the proportion of cells in G, suggesting that propionic acid causes a cell cycle arrest in yeast. Finally, we show that the reduction of MTT is attenuated in yeast cells treated with propionic acid, indicating that propionic acid disrupts cellular respiration. Understanding the effects of propionic acid on the yeast cell may aid in assessing the broader utility of this compound.
我们之前发现丙酸是一种具有杀菌活性的微生物产生的挥发性有机化合物，能对抗几种致病真菌。本研究旨在通过研究该化合物对酵母细胞的一些影响，更好地了解丙酸是如何影响真菌的。
我们表明，丙酸导致酵母细胞内 lucifer yellow 的摄取量显著增加，这与内吞作用增强一致。此外，我们还通过碘化丙啶测定法表明，丙酸处理会导致酵母细胞中 G 期比例显著增加，而 G 期比例显著降低，表明丙酸会导致酵母细胞周期停滞。最后，我们表明，用丙酸处理的酵母细胞中 MTT 的还原减少，表明丙酸破坏了细胞呼吸。了解丙酸对酵母细胞的影响可能有助于评估该化合物的更广泛应用。
