Abreu S L, Kaplan P
J Interferon Res. 1984 Spring;4(2):161-6. doi: 10.1089/jir.1984.4.161.
Incubation of rat fibroblasts (RFA-1) with theophylline after Newcastle disease virus (NDV) induction, increased rat fibroblast interferon (RfIFN) yields 3 to 10-fold. However, incubation with butyric acid for 24 or 48 h before NDV addition resulted in no change or decrease in IFN production. Combined treatment with butyric acid and theophylline did not improve the yields obtained with the methylxanthine alone. Production of IFN by RFA-1 cells was the same in serum-free and in serum-containing media. These findings will facilitate RfIFN production and purification.
在新城疫病毒（NDV）诱导后用茶碱孵育大鼠成纤维细胞（RFA-1），可使大鼠成纤维细胞干扰素（RfIFN）产量提高3至10倍。然而，在添加NDV之前用丁酸孵育24或48小时，导致干扰素产量没有变化或下降。丁酸和茶碱联合处理并没有提高单独使用甲基黄嘌呤所获得的产量。RFA-1细胞在无血清和含血清培养基中产生的干扰素相同。这些发现将有助于RfIFN的生产和纯化。
