Metal-organic frameworks or MOFs are coordination polymers consisting of cationic metal centers liked by ligands. These coordination polymers have repeating entities that extend in one, two, or three dimensions through various Metal-ligand covalent bonds. The structural diversity of MOFs allows for the fine-tuning of properties like pore size, stability, and functionality, making them ideal for a wide range of industrial, environmental, and biomedical applications. Basolite C-300, HKUST-1 or [Cu(btc)(HO)], is one of the most studied three-dimensional porous frameworks. It is a commercially available MOF, easily produced under laboratory conditions. Its unique cubic structure, with multiple pore and adsorption sites, enhances its properties. This article reviews the conventional, new, and non-conventional methods of MOF and Basolite C-300 synthesis. In addition, the structural and spectral characterization of Basolite C-300 and its analogues is described, using spectroscopic and complementary multi-techniques to obtain fundamental knowledge about their structure. Finally, the applications of Basolite C-300 and similar MOFs are discussed, emphasizing their importance in industry and materials, technologies aimed at addressing global environmental and energy-related challenges, and biomedical applications.