Condurso Concetta, Merlino Maria, Miller Anthea, Di Rosa Ambra Rita, Accetta Francesca, Leonardi Michelangelo, Cicero Nicola, Gervasi Teresa
Department of Veterinary Sciences, University of Messina, 98168 Messina, Italy.
Department of Biomedical and Dental Sciences and Morphofunctional Imaging, University of Messina, 98168 Messina, Italy.
Foods. 2025 Jun 19;14(12):2148. doi: 10.3390/foods14122148.
Consumer demand for plant-based functional foods, especially probiotic beverages, has increased due to their health benefits and suitability as dairy-free alternatives. This study assessed, through a factorial combination, the stability of plant-based extracts (avocado, ginger, and tropical) individually inoculated with three commercial strains (, , ) and stored under refrigerated conditions during both primary (PSL) and secondary shelf life (SSL). Product shelf life was defined by probiotic viability, considering the functional threshold (≥6 log CFU/mL), which was maintained across all formulations throughout the storage period. Physicochemical parameters, including pH, titratable acidity, and colour, as well as volatile profile, remained stable, with only minor variations depending on the matrix and bacterial strain. Sensory evaluations (triangle and acceptability tests) confirmed that the probiotic juices were acceptable to consumers. Overall, the results demonstrate the feasibility of producing non-fermented, plant-based probiotic beverages that retain their functional properties and meet consumer sensory expectations, offering a promising alternative for vegan and lactose-intolerant individuals.
由于植物基功能性食品,特别是益生菌饮料具有健康益处且适合作为无乳制品替代品,消费者对其需求有所增加。本研究通过析因组合评估了分别接种三种商业菌株(、、)的植物提取物(鳄梨、姜和热带水果)的稳定性,并在冷藏条件下在初级货架期(PSL)和次级货架期(SSL)进行储存。产品货架期由益生菌活力定义,考虑到功能阈值(≥6 log CFU/mL),在整个储存期内所有配方均保持该阈值。包括pH值、可滴定酸度和颜色在内的理化参数以及挥发性成分保持稳定,仅根据基质和菌株略有变化。感官评价(三角试验和可接受性测试)证实益生菌果汁为消费者所接受。总体而言,结果表明生产保留其功能特性并满足消费者感官期望的非发酵植物基益生菌饮料是可行的,为纯素食者和乳糖不耐受者提供了一个有前景的选择。