Tian Jieyun, Ma Yao, Chen Yabing, Chen Xue, Wei Anzhi
College of Forestry, Northwest A&F University, Xianyang, China.
School of Agricultural Sciences, Zhengzhou University, Zhengzhou, China.
Front Plant Sci. 2022 Apr 29;13:847202. doi: 10.3389/fpls.2022.847202. eCollection 2022.
Plant growth and survival in nature, its growth process, will be affected by various factors from the environment, among which temperature has a greater impact. In recent years, extreme weather has frequently appeared, and the growth of crops has been increasingly affected by the environment. As an important flavoring and Chinese herbal medicine crop, is also facing the harm of low-temperature stress. Plant hormones play a vital role in the response of plants to low temperatures. In this study, ultra-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to determine the hormone components of cold-tolerant and cold-sensitive varieties of . Combined with chemometric analysis and weighted gene co-expression network analysis (WGCNA), the hormone component differences and hormone response strategies of under low-temperature stress were comprehensively studied. The results showed that 45 hormones were detected in . Among them, there were 7 kinds of components with high content and were detected in both two varieties. At the late stage of low-temperature stress, the contents of abscisic acid (ABA) and ABA-glucosyl ester (ABA-GE) in Fuguhuajiao (FG) were significantly increased, and the latter served as the storage of the former to supplement the active ABA. Orthogonal partial least squares discriminant analysis (OPLS-DA) found that indole-3-carboxylic acid (ICA), indole-3-carboxaldehyde (ICAld), meta-Topolin riboside (mTR), cis-Zeatin-O-glucoside riboside (cZROG), and N6-isopentenyladenosine (IPR) in FG were the upregulated important difference components, and IPR and 2-methylthio-cis-zeatin riboside (2MeScZR) in Fengxiandahongpao (FX) were the upregulated important difference components. There were common crossing points and independent response pathways in response to low temperature in two varieties. WGCNA analysis found that the main hormone components were associated with multiple metabolic pathways including carbon, fatty acid, amino acid, and sugar metabolism, indicating that hormone regulation plays an important role in the response of to low temperature. This study clarified the hormone response mechanism of under low-temperature stress and provided a reference and basis for further improving the cold resistance of and cultivating new varieties.
植物在自然环境中的生长与存活，即其生长过程，会受到来自环境的各种因素影响，其中温度的影响较大。近年来，极端天气频繁出现，农作物的生长越来越受到环境的影响。作为一种重要的调味作物和中药材作物，[具体作物名称]也面临着低温胁迫的危害。植物激素在植物对低温的响应中起着至关重要的作用。在本研究中，采用超高效液相色谱 - 串联质谱法（LC - MS/MS）测定了[具体作物名称]耐寒和冷敏品种的激素成分。结合化学计量学分析和加权基因共表达网络分析（WGCNA），全面研究了[具体作物名称]在低温胁迫下的激素成分差异和激素响应策略。结果表明，在[具体作物名称]中检测到45种激素。其中，有7种含量较高的成分，且在两个品种中均能检测到。在低温胁迫后期，伏虎花胶（FG）中脱落酸（ABA）和ABA - 葡萄糖酯（ABA - GE）的含量显著增加，后者作为前者的储存形式以补充活性ABA。正交偏最小二乘法判别分析（OPLS - DA）发现，FG中的吲哚 - 3 - 羧酸（ICA）、吲哚 - 3 - 甲醛（ICAld）、间 - 托布津核苷（mTR）、顺式玉米素 - O - 葡萄糖苷核糖苷（cZROG）和N6 - 异戊烯基腺苷（IPR）是上调的重要差异成分，凤仙大红袍（FX）中的IPR和2 - 甲硫基 - 顺式玉米素核糖苷（2MeScZR）是上调的重要差异成分。两个品种在对低温的响应中存在共同交叉点和独立响应途径。WGCNA分析发现，主要激素成分与包括碳、脂肪酸、氨基酸和糖代谢在内的多个代谢途径相关，表明激素调节在[具体作物名称]对低温的响应中起着重要作用。本研究阐明了[具体作物名称]在低温胁迫下的激素响应机制，为进一步提高[具体作物名称]的抗寒性和培育新品种提供了参考和依据。
