Choneva Mariya, Bivolarska Anelia, Gyuzeleva Donika, Turiyski Valentin, Stoyanov Plamen, Mladenova Tsvetelina, Todorov Krasimir, Mladenov Rumen, Prissadova Natalia, Ardasheva Raina, Yotov Viktor, Denev Petko, Topalova-Shishmanova Aleksandrina, Bivolarski Stoyan, Dimov Ivica
Department of Medical Biochemistry, Faculty of Pharmacy, Medical University of Plovdiv, 15A Vasil Aprilov Blvd., 4002 Plovdiv, Bulgaria.
Department of Botany and Biological Education, Faculty of Biology, University of Plovdiv "Paisii Hilendarski", 24 Tsar Assen Str., 4000 Plovdiv, Bulgaria.
Life (Basel). 2025 Jun 12;15(6):948. doi: 10.3390/life15060948.
The genus (Lamiaceae) is widely used in traditional medicine. While some representatives of the genus have been well investigated, the biological activity of others remains largely unknown. The aim of the current study was to assess the in vitro antioxidant potential and the effect on the reactivity of isolated rat gastric smooth muscles (SM) of aqueous extracts of inflorescences, stems and leaves, and as a whole herb. The antioxidant activity was analyzed through multiple spectrophotometric and fluorometric assays. The effect on SM reactivity was determined by the treatment of excised gastric muscles of 10 male Whistar rats with the plant extracts alone or successive to 1,1-dimethyl-4-diphenylacetoxypiperidinium iodide, ketanserin, verapamil, and acetylcholine. According to the obtained results, the leaf extract exhibited the greatest antioxidant potential, followed by the one. The SM reactivity analysis revealed that the treatment with all four extracts induced a dose-dependent contractile response with predominant cholinergic character. However, activation of serotoninergic and/or dopaminergic pathways was also observed. Furthermore, when applied after verapamil, the extracts showed a SM relaxant effect. The discovered biological activity will serve as a basis for future analyses through which the therapeutic effect of the plants will be investigated.
属（唇形科）在传统医学中广泛应用。虽然该属的一些代表植物已得到充分研究，但其他植物的生物活性仍 largely unknown。本研究的目的是评估该属植物花序、茎和叶以及全草水提取物的体外抗氧化潜力及其对分离的大鼠胃平滑肌（SM）反应性的影响。通过多种分光光度法和荧光法分析抗氧化活性。通过单独用植物提取物或在1,1 - 二甲基 - 4 - 二苯基乙酰氧基哌啶碘化物、酮色林、维拉帕米和乙酰胆碱之后处理10只雄性Wistar大鼠的离体胃肌来确定对SM反应性的影响。根据所得结果，该属植物叶提取物表现出最大的抗氧化潜力，其次是该属植物全草提取物。SM反应性分析表明，用所有四种提取物处理均诱导出具有主要胆碱能特征的剂量依赖性收缩反应。然而，也观察到了5 - 羟色胺能和/或多巴胺能途径的激活。此外，在维拉帕米之后应用时，提取物显示出SM舒张作用。所发现的生物活性将作为未来分析的基础，通过这些分析来研究这些植物的治疗效果。
