Fungal Biodegradation of Amlodipine and Lisinopril by Penicillium citrinum: Towards a Sustainable Approach to Pharmaceutical Wastewater Treatment.

Concerns are rising globally about the contamination caused by pharmaceutical waste, where improper disposal of unused medications can harm the environment and pose risks to public health. Household pharmaceutical waste needs careful management to mitigate its impact, as medicines disposed in sinks or flushed down in toilets can end up in sewage systems and eventually contaminate waterways. This study aims to address these concerns by investigating the degradation of most commonly used drugs for hypertension amlodipine besylate and lisinopril, using Penicillium citrinum. The fungal isolate tolerated 100 mg L of amlodipine and lisinopril and degraded 96.4% and 95.7% within 7th and 8th d, respectively, and exhibited enzyme activities such as lignin peroxidase, manganese peroxidase, and laccase, which played a crucial role in amlodipine and lisinopril degradation. Notably, the degradation process yields novel byproducts which was not previously identified in any scientific studies. The degradation process was confirmed through various analytical techniques including High Performance Liquid Chromatography (HPLC), Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR), Nuclear Magnetic Resonance (NMR) and Liquid Chromatography Mass Spectroscopy (LCMS). The LCMS analysis showed the presence of three degradation products of amlodipine; Octadecanal, dodecane-1,1-diol and Pentane-1, 1-diol. And for lisinopril two byproducts (3-ethyloctane and Diethoxymethane) were identified. The degradation satisfies the first order kinetics model with a higher R value of 0.95 and 0.963. The results indicate that the strain was successful in removing amlodipine and lisinopril efficiently.