Ferrer Marianelly Fernandez, Retuerto Mauricio, Thavamani Aravind, San Valentin Erin Marie, Sferra Thomas J, Ghannoum Mahmoud, Sankararaman Senthilkumar
Division of Pediatric Gastroenterology, Hepatology & Nutrition, Department of Pediatrics, UH Rainbow Babies and Children's Hospital, Cleveland, OH 44106, USA.
Department of Pediatrics, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA.
Nutrients. 2025 Jun 17;17(12):2018. doi: 10.3390/nu17122018.
Diet is known to influence the composition of the gut microbiome. For patients who require enteral feeding, there has been a growing popularity of using blenderized tube feeds (BTFs) as an alternative to standard enteral formula (SEF). There is limited literature exploring the impact of BTFs on the gut microbiome. Twenty-eight patients 1 to 22 years of age who received their nutrition via gastrostomy tube for over 4 weeks were included and participants were divided into BTF and SEF groups. Demographics and clinical information were collected from the medical records, and all legal guardians completed a semi-structured interview using a questionnaire. 16SrRNA sequencing was used for bacteriome analysis. Eleven patients in the BTF group and seventeen in the SEF group were included. No significant differences in the demographics were noted. Patients on BTFs had no emesis compared to seven (41%) in the SEF group, = 0.02. There were no significant differences in other clinical characteristics and comorbidities. No significant differences in the gut microbiome between the groups were noted for alpha and beta diversities, richness, and evenness (at both genus and species levels). Differential abundance analysis showed only a few significant differences between the groups at all reported taxonomic levels. Patients on BTFs had a significantly decreased prevalence of emesis compared to the SEF group. No significant differences in the microbiome between the groups were noted for alpha and beta diversities, richness, and evenness. Prospective studies are recommended to verify our preliminary data and further evaluate the implications of our study results.
众所周知，饮食会影响肠道微生物群的组成。对于需要肠内喂养的患者，使用搅拌型管饲（BTF）作为标准肠内配方（SEF）的替代品越来越普遍。探索BTF对肠道微生物群影响的文献有限。纳入了28名1至22岁通过胃造口管接受营养超过4周的患者，并将参与者分为BTF组和SEF组。从病历中收集人口统计学和临床信息，所有法定监护人使用问卷完成了半结构化访谈。采用16SrRNA测序进行细菌群落分析。BTF组纳入11名患者，SEF组纳入17名患者。两组在人口统计学上无显著差异。BTF组患者无呕吐，而SEF组有7名（41%）患者呕吐，P = 0.02。其他临床特征和合并症无显著差异。两组之间在α和β多样性、丰富度和均匀度（属和种水平）方面的肠道微生物群无显著差异。差异丰度分析显示，在所有报告的分类水平上，两组之间仅有少数显著差异。与SEF组相比，BTF组患者呕吐的发生率显著降低。两组之间在微生物群的α和β多样性、丰富度和均匀度方面无显著差异。建议进行前瞻性研究以验证我们的初步数据，并进一步评估我们研究结果的意义。
