Herrera da Silva Joao P, Rossow Stephanie, Paploski Igor A D, Kikuti Mariana, Corzo Cesar A, VanderWaal Kimberly
Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55455, USA.
Viruses. 2025 Jun 18;17(6):863. doi: 10.3390/v17060863.
Porcine Reproductive and Respiratory Syndrome Virus 2 (PRRSV-2) represents one of the greatest threats to global pork production. Increased incidence of a genetic variant of Porcine Reproductive and Respiratory Syndrome Virus (variant 1H.18) was recently reported in the Midwestern USA Sequence comparisons in the ORF5 region indicate that 1H.18 was closely related to both sub-lineages L1H and L1C. To expand our understanding and attempt to elucidate the origin of the 1H.18, we sequenced a near-complete genome, covering all coding regions, and investigated the occurrence of recombination events that may have contributed to the emergence of the new variant. At least six distinct recombination events were identified across the coding portion of the genome. Evidence of recombination in the ORF5 region between variants 1H.31 and 1C.3 was detected. Our results suggest a likely origin of 1H.18 driven by recombination.
猪繁殖与呼吸综合征病毒2型（PRRSV - 2）是全球猪肉生产面临的最大威胁之一。最近美国中西部地区报告了猪繁殖与呼吸综合征病毒一种基因变体（1H.18变体）的发病率增加。开放阅读框5（ORF5）区域的序列比较表明，1H.18与L1H和L1C两个亚谱系密切相关。为了加深我们的理解并试图阐明1H.18的起源，我们对一个几乎完整的基因组进行了测序，覆盖所有编码区域，并研究了可能导致新变体出现的重组事件的发生情况。在基因组的编码部分至少鉴定出六个不同的重组事件。在1H.31和1C.3变体之间的ORF5区域检测到了重组证据。我们的结果表明，重组可能是1H.18的起源原因。
