Zhu Mo, Zhang Fuhai, Qiu Zongbo, Zhao Sujing, Gao Shiqiang
College of Life Sciences Henan Normal University Xinxiang P.R. China.
Henan Center for Outstanding Overseas Scientists Henan Normal University Xinxiang P.R. China.
Food Sci Nutr. 2025 Jun 28;13(7):e70396. doi: 10.1002/fsn3.70396. eCollection 2025 Jul.
The fungal developmental processes are orchestrated by white light. Despite the genome assembly of being available, the underlying molecular mechanisms of the white light-mediated developments of remain obscure. It was found that white light impaired mycoparasitic activities against the wheat powdery mildew fungus and infection processes on tomato fruits in . In vitro and in vivo, white light significantly impaired colony expansion and dramatically increased conidiation of . RNA-seq analysis of conidia was profiled to illustrate the light-mediated expression of genes. A total of 153 and 666 differentially expressed genes were identified between conidia treated with or without white light at 48- and 96-h post inoculation (hpi). Among genome-wide identified effectors, 8 and 36 effectors were differentially regulated by white light at 48 and 96 hpi, respectively. The core effectors, Tro004101, Tro006854, Tro008316, and Tro004104 were commonly downregulated by white light. Notably, white light regulated gene expression in key metabolic pathways including tryptophan metabolism (3 genes) and tyrosine metabolism (5 genes), as well as the HOG-MAPK signaling cascade. These results demonstrated that white light-compromised mycoparasitic and infection activities might be achieved by regulating specific effector expression and differentially modulating metabolism and HOG-MAPK pathways. The genes detected by our transcriptome analysis may be crucial for mycoparasitism and infection by and thus serve as targets for future functional analysis. Our findings provide new insights into the white light-orchestrated developments of an important agricultural and economical fungus and will potentially support efforts for the study of fungal effectors.
真菌的发育过程由白光调控。尽管已有其基因组组装结果，但白光介导的其发育的潜在分子机制仍不清楚。研究发现，白光会损害其对小麦白粉病菌的重寄生活性以及在番茄果实上的侵染过程。在体外和体内，白光显著抑制其菌落扩展，并显著增加其分生孢子形成。对其分生孢子进行RNA测序分析以阐明光介导的基因表达。在接种后48小时（hpi）和96小时，分别在有或无白光处理的分生孢子之间鉴定出总共153个和666个差异表达基因。在全基因组鉴定的效应子中，分别有8个和36个效应子在48 hpi和96 hpi时受到白光的差异调节。核心效应子Tro004101、Tro006854、Tro008316和Tro004104通常被白光下调。值得注意的是，白光调节关键代谢途径中的基因表达，包括色氨酸代谢（3个基因）和酪氨酸代谢（5个基因），以及HOG-MAPK信号级联。这些结果表明，白光损害的其重寄生和侵染活性可能是通过调节特定效应子表达以及差异调节代谢和HOG-MAPK途径来实现的。我们通过转录组分析检测到的基因可能对其重寄生和侵染至关重要，因此可作为未来功能分析的靶点。我们的研究结果为一种重要的农业和经济真菌的白光调控发育提供了新的见解，并可能为真菌效应子的研究提供支持。
