Graciano-España María Carmen, Barnhart Kurt, Gonzalez-Monfort Marta, Arenas-Barrero Marta, Legro Richard S, Thomas Tracey R, Rush Margaret A, Vilella Felipe, Fernández-Sánchez Manuel, Simon Carlos, Moreno Inmaculada
Carlos Simon Foundation, Valencia, Spain; INCLIVA Biomedical Research Institute, Valencia, Spain; Pediatrics, Obstetrics and Gynecology Department, Faculty of Medicine, University of Valencia, Valencia, Spain.
Penn Medicine Women's Health Clinical Research Center, University of Pennsylvania, Philadelphia, Pennsylvania.
Fertil Steril. 2025 Jun 28. doi: 10.1016/j.fertnstert.2025.06.035.
To evaluate the presence of Fusobacterium spp. and Fusobacterium nucleatum in eutopic endometrial samples from women with endometriosis compared with controls, and assess their association with the disease.
Retrospective case-control study.
Ninety-two women (55 endometriosis cases and 37 controls) aged 18-44 years undergoing gynecologic endoscopy for endometriosis and/or benign conditions at University of Pennsylvania, Endomarker Study (PMID: 29524590).
DNA extraction from eutopic endometrial samples using QIAamp DNA Microbiome and IndiSpin Pathogen Kits. Quantification of Fusobacterium spp. and F. nucleatum by quantitative PCR using genus- and species-specific primers.
MAIN OUTCOME MEASURE(S): Relative abundance of Fusobacterium spp. and F. nucleatum in cases vs. controls, analyzed using the ΔCt method.
No significant difference in Fusobacterium spp. or F. nucleatum abundance was observed between cases and controls (P = .258 for genus-specific primers, P = .738 for species-specific primers). Subgroup analysis by disease severity (minimal-mild: n = 42; moderate-severe: n = 13) also showed no significant differences (Fusobacterium spp.: P = .1465; F. nucleatum: P = .2936).
Fusobacterium spp. is not differentially present in eutopic endometrium of women with endometriosis, regardless of disease severity according to revised American Society for Reproductive Medicine classification. This contrasts with prior findings in eutopic endometrium in patients with ovarian endometriosis, suggesting that Fusobacterium has limited diagnostic or prognostic value in endometriosis.
评估与对照组相比，子宫内膜异位症患者在位内膜样本中梭杆菌属及具核梭杆菌的存在情况，并评估它们与该疾病的关联。
回顾性病例对照研究。
92名年龄在18 - 44岁之间的女性（55例子宫内膜异位症患者和37名对照），她们在宾夕法尼亚大学因子宫内膜异位症和/或良性疾病接受妇科内镜检查（子宫内膜标记物研究，PMID：29524590）。
使用QIAamp DNA微生物组试剂盒和IndiSpin病原体试剂盒从在位内膜样本中提取DNA。使用属特异性和种特异性引物通过定量PCR对梭杆菌属和具核梭杆菌进行定量。
使用ΔCt法分析病例组与对照组中梭杆菌属和具核梭杆菌的相对丰度。
病例组和对照组之间梭杆菌属或具核梭杆菌的丰度没有显著差异（属特异性引物：P = 0.258；种特异性引物：P = 0.738）。按疾病严重程度进行的亚组分析（轻度 - 中度：n = 42；重度：n = 13）也未显示出显著差异（梭杆菌属：P = 0.1465；具核梭杆菌：P = 0.2936）。
根据美国生殖医学学会修订分类，无论疾病严重程度如何，子宫内膜异位症患者在位内膜中梭杆菌属的存在无差异。这与卵巢子宫内膜异位症患者在位内膜的先前研究结果形成对比，表明梭杆菌在子宫内膜异位症中具有有限的诊断或预后价值。
