子宫内膜微生物组的组成与不孕患者的生殖结局有关。
Endometrial microbiota composition is associated with reproductive outcome in infertile patients.
机构信息
Igenomix Foundation, INCLIVA Health Research Institute, Valencia, Spain.
Igenomix R&D, Valencia, Spain.
出版信息
Microbiome. 2022 Jan 4;10(1):1. doi: 10.1186/s40168-021-01184-w.
BACKGROUND
Previous evidence indicates associations between the female reproductive tract microbiome composition and reproductive outcome in infertile patients undergoing assisted reproduction. We aimed to determine whether the endometrial microbiota composition is associated with reproductive outcomes of live birth, biochemical pregnancy, clinical miscarriage or no pregnancy.
METHODS
Here, we present a multicentre prospective observational study using 16S rRNA gene sequencing to analyse endometrial fluid and biopsy samples before embryo transfer in a cohort of 342 infertile patients asymptomatic for infection undergoing assisted reproductive treatments.
RESULTS
A dysbiotic endometrial microbiota profile composed of Atopobium, Bifidobacterium, Chryseobacterium, Gardnerella, Haemophilus, Klebsiella, Neisseria, Staphylococcus and Streptococcus was associated with unsuccessful outcomes. In contrast, Lactobacillus was consistently enriched in patients with live birth outcomes.
CONCLUSIONS
Our findings indicate that endometrial microbiota composition before embryo transfer is a useful biomarker to predict reproductive outcome, offering an opportunity to further improve diagnosis and treatment strategies. Video Abstract.
背景
先前的证据表明，不孕患者在接受辅助生殖时，女性生殖道微生物组的组成与生殖结局有关。我们旨在确定子宫内膜微生物组的组成是否与活产、生化妊娠、临床流产或无妊娠的生殖结局相关。
方法
在这里，我们进行了一项多中心前瞻性观察研究，使用 16S rRNA 基因测序来分析 342 名无症状感染的不孕患者在胚胎移植前的子宫内膜液和活检样本，这些患者正在接受辅助生殖治疗。
结果
由 Atopobium、Bifidobacterium、Chryseobacterium、Gardnerella、Haemophilus、Klebsiella、Neisseria、Staphylococcus 和 Streptococcus 组成的失调子宫内膜微生物组谱与不良结局相关。相比之下，Lactobacillus 在活产结局的患者中始终富集。
结论
我们的研究结果表明，胚胎移植前的子宫内膜微生物组组成是预测生殖结局的有用生物标志物，为进一步改善诊断和治疗策略提供了机会。视频摘要。
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