Megli Christina J, Zack Rebecca K, McGaughey Jackson J, Hoehl Ryan M, Snisky Taylor, Hartman Amy L, McMillen Cynthia M
University of Pittsburgh, School of Medicine, Division of Maternal-Fetal Medicine, Division of Reproductive Infectious Disease, Department of Obstetrics, Gynecology and Reproductive Sciences, Pittsburgh, PA, USA.
Magee-Womens Research Institute, Pittsburgh, PA, USA.
Nat Commun. 2025 Jul 1;16(1):6040. doi: 10.1038/s41467-025-61138-w.
Clinical and epidemiologic evidence from the 2023-24 outbreak of Oropouche virus (OROV) has demonstrated increased severity in clinical disease and adverse pregnancy outcomes including miscarriage, stillbirth, and neonatal demise. Serological evidence suggests vertical transmission of OROV may be responsible. OROV has not been studied in the context of pregnancy and has an unknown ability to infect the relevant tissues of the maternal-fetal interface; therefore, the mechanisms of vertical transmission are unknown. We use human cytotrophoblast and syncytiotrophoblast stem cell cultures, polarized trophoblast stem cell organoids, and placenta explants to demonstrate that OROV (BeAn19991) infects and replicates in human tissues of the maternal-fetal interface including cytotrophoblasts and the microbial-resistant cell, syncytiotrophoblast. Viral replication is robust within the first 24 hour post infection and infection may be dependent on gestational age. These data indicate tissues at the maternal-fetal interface are susceptible to OROV infection and may facilitate vertical transmission, leading to adverse pregnancy outcomes.
2023 - 2024年奥罗普切病毒（OROV）疫情的临床和流行病学证据表明，临床疾病的严重程度增加，不良妊娠结局增多，包括流产、死产和新生儿死亡。血清学证据表明，OROV的垂直传播可能是原因所在。尚未在妊娠背景下对OROV进行研究，其感染母胎界面相关组织的能力未知；因此，垂直传播的机制尚不清楚。我们使用人细胞滋养层细胞和合体滋养层干细胞培养物、极化滋养层干细胞类器官以及胎盘外植体来证明，OROV（BeAn19991）可在母胎界面的人体组织中感染和复制，包括细胞滋养层细胞和具有微生物抗性的合体滋养层细胞。病毒复制在感染后的最初24小时内很强劲，感染可能取决于胎龄。这些数据表明，母胎界面的组织易受OROV感染，并可能促进垂直传播，导致不良妊娠结局。
