Koltai Erika, Mozaffaritabar Soroosh, Zhou Lei, Kolonics Attila, Koike Atsuko, Tanisawa Kumpei, Park Jonguk, Torma Ferenc, Radak Zsolt
Research Institute of Sport Science, Hungarian University of Sport Science, Alkotas Street, 42-46, Budapest, 1123, Hungary.
Faculty of Sport Sciences, Waseda University, Tokorozawa, Japan.
Sci Rep. 2025 Jul 1;15(1):20527. doi: 10.1038/s41598-025-05594-w.
In this study, we investigated the potential relationship between the mitochondrial network and the microbiome using wild-type and skeletal muscle-specific PGC-1α (Pparg coactivator 1 alpha) overexpressing mice, both with and without exercise training. Basal PGC-1α levels were significantly higher in the skeletal muscle (J Physiol Biochem 80:329-335, 2024. https://doi.org/10.1007/s13105-024-01006-1 ) and, notably, in the colon, which is anatomically proximal to the microbiome. However, no significant changes were observed in cell signaling or mitochondria-related proteins within the colon. On the other hand, mitochondrial H₂O₂ production in the colon decreased in the PGC-1α overexpressing group. The relative abundance of several bacterial taxa differed between wild-type and PGC-1α overexpressing groups at baseline condition, indicating a shift in the microbiome milieu probably to cope with the increased metabolism, enhanced short-chain fatty acid utilization, and improved endurance capacity. Ten weeks of exercise training differentially modulated the host microbiome in PGC-1α overexpressing and wild-type mice, facilitating adaptations to a broad range of exercise-induced challenges. The results of this study provide new insights into the possible cross-talk between mitochondria and the microbiome.
在本研究中，我们使用野生型小鼠以及骨骼肌特异性过表达PGC-1α（过氧化物酶体增殖物激活受体γ共激活因子1α）的小鼠，研究了线粒体网络与微生物群之间的潜在关系，这些小鼠均进行或未进行运动训练。基础PGC-1α水平在骨骼肌中显著更高（《生理学与生物化学杂志》80:329 - 335，2024年。https://doi.org/10.1007/s13105-024-01006-1 ），值得注意的是，在结肠中也显著更高，结肠在解剖学上与微生物群相邻。然而，在结肠内的细胞信号传导或线粒体相关蛋白方面未观察到显著变化。另一方面，过表达PGC-1α的组中结肠内的线粒体H₂O₂产生减少。在基线条件下，野生型和过表达PGC-1α的组之间几种细菌类群的相对丰度有所不同，这表明微生物群环境发生了变化，可能是为了应对增加的代谢、增强的短链脂肪酸利用以及提高的耐力能力。十周的运动训练对过表达PGC-1α的小鼠和野生型小鼠的宿主微生物群产生了不同的调节作用，促进了对广泛的运动诱导挑战的适应。本研究结果为线粒体与微生物群之间可能的相互作用提供了新的见解。
