Chang Runze, Wang Heng, Lu Chuanlong, Chen Jinshan, Li Yaling, Qiao Maolin, Gao Siqi, Li Lizheng, Fan Keyi, Zhang Ruijing, Dong Honglin
The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, China.
Centre for Transplant and Renal Research, Westmead Institute for Medical Research, The University of Sydney, Sydney, NSW 2145, Australia.
iScience. 2025 Jun 5;28(7):112828. doi: 10.1016/j.isci.2025.112828. eCollection 2025 Jul 18.
Abdominal aortic aneurysm (AAA) is a serious cardiovascular disease associated with chronic inflammation. The purpose of this study was to use the Olink proteomics to reveal serum inflammatory markers in AAA. We examined the expression levels of 92 inflammation-related proteins in patients with AAA ( = 18) and healthy individuals ( = 10) using the Olink proximity extension assay (PEA) inflammatory plate. Olink proteomics identified 38 differential proteins. Combined analysis of Olink proteomics and GSE183464 showed interleukin-6 (IL-6) and tumor necrosis factor receptor superfamily member 9 (TNFRSF9) were upregulated at both gene and protein levels in AAA patients. The ELISA results were consistent with the Olink proteomics results, and the receiver operating characteristic (ROC) curve analysis revealed that the binding of TNFRSF9 and IL-6 has high diagnostic value (Olink AUC = 0.9056; ELISA AUC = 0.950). Subsequently, elevated TNFRSF9 expression in AAA was confirmed by animal models, suggesting that TNFRSF9 may serve as a potential biomarker for AAA.
腹主动脉瘤（AAA）是一种与慢性炎症相关的严重心血管疾病。本研究的目的是使用欧林蛋白质组学技术揭示AAA患者血清中的炎症标志物。我们使用欧林邻位延伸分析（PEA）炎症板检测了18例AAA患者和10例健康个体中92种炎症相关蛋白的表达水平。欧林蛋白质组学技术鉴定出38种差异蛋白。欧林蛋白质组学技术与GSE183464的联合分析表明，AAA患者中白细胞介素-6（IL-6）和肿瘤坏死因子受体超家族成员9（TNFRSF9）在基因和蛋白水平均上调。酶联免疫吸附测定（ELISA）结果与欧林蛋白质组学技术结果一致，受试者工作特征（ROC）曲线分析显示，TNFRSF9与IL-6的结合具有较高的诊断价值（欧林蛋白质组学技术曲线下面积[AUC]=0.9056；ELISA AUC=0.950）。随后，动物模型证实了AAA中TNFRSF9表达升高，这表明TNFRSF9可能是AAA的潜在生物标志物。
