Patır İlkyaz, Karkar Büşra, Şahin Saliha
Department of Chemistry, Faculty of Science and Arts Bursa Uludag University Bursa Türkiye.
Food Sci Nutr. 2025 Jul 1;13(7):e70438. doi: 10.1002/fsn3.70438. eCollection 2025 Jul.
Bioaccessibility is a significant problem hindering dietary polyphenols' beneficial effect on human health. Plants, such as spices, have a high polyphenol content, but the human body can only benefit from the part absorbed from the intestine. Hance is also a spice with high polyphenol content, but its phenolic compounds have low bioaccessibility with low solubility, stability, and poor intestinal absorption. Although various carrier systems have been developed to overcome this situation, the performance of chitosan-sodium alginate coated liposomes was evaluated for the first time in this study. It was demonstrated that in contrast to various carrier systems developed, double-coated liposomes preserve the active ingredients and increase their bioaccessibility in environments with different pH, enzyme, and salt conditions. For the enhancement of the bioaccessibility of its phenolic compounds, Hance extract was encapsulated in dual-coated liposomes. For this purpose, chromatographic and spectroscopic analysis of Hance extract was performed. The dual-coated liposomes were produced and characterized, and the in vitro release profile and bioaccessibility were investigated. The significant differences between the results were statistically analyzed. The liposomes were produced with 93.19% ± 0.02% encapsulation efficiency and a size of 155 ± 22.61 nm, zeta potential of -40.97 ± 13.41 mV. The bioaccessibility of galangin in Hance extract was 23.87% ± 0.24%, and in dual-coated liposomes was 73.65% ± 1.70%. By virtue of the developed system, liposomes loaded with Hance extract resisted gastrointestinal conditions and increased its bioaccessibility approximately threefold by slowing the release of the extract. The results are statistically significant.
生物可及性是阻碍膳食多酚对人体健康产生有益作用的一个重要问题。植物，如香料，含有高含量的多酚，但人体只能从肠道吸收的部分中获益。山柰也是一种多酚含量高的香料，但其酚类化合物的生物可及性较低，具有低溶解度、稳定性差和肠道吸收不良的特点。尽管已经开发了各种载体系统来克服这种情况，但本研究首次评估了壳聚糖-海藻酸钠包衣脂质体的性能。结果表明，与已开发的各种载体系统相比，双层包衣脂质体能够保留活性成分，并在不同pH值、酶和盐条件的环境中提高其生物可及性。为了提高其酚类化合物的生物可及性，将山柰提取物包封在双层包衣脂质体中。为此，对山柰提取物进行了色谱和光谱分析。制备并表征了双层包衣脂质体，并研究了其体外释放曲线和生物可及性。对结果之间的显著差异进行了统计分析。制备的脂质体包封率为93.19%±0.02%，粒径为155±22.61nm，ζ电位为-40.97±13.41mV。山柰提取物中高良姜素的生物可及性为23.87%±0.24%，在双层包衣脂质体中为73.65%±1.70%。借助所开发的系统，负载山柰提取物的脂质体能够抵抗胃肠道环境，并通过减缓提取物的释放使其生物可及性提高约三倍。结果具有统计学意义。
